沙鼠短暂性脑缺血及西比灵干预后脑内Smad2和Smad4表达的研究-神经病学专业论文.docxVIP

硕t学位论文 硕t学位论文 中文摘要 摘要 目的：研究沙鼠短暂性脑缺血及西比灵干预后脑内Smad2和 Smad4蛋白的表达。 方法：采用夹闭双侧颈总动脉的方法制作沙鼠脑缺妞再灌注模 型；西比灵治疗组的沙鼠在实验前喂食西比灵：各组沙鼠在相应的时 间点处死，取前脑组织，用免疫组化法检测脑内Smad2和Smad4蛋 白的表达；结果用Spssll．0统计分析。 结果：60只沙鼠脑组织的神经元和神经胶质细胞内均有Smad2 和Smad4蛋白阳性表达，且Smad4蛋白表达均显著强于Smad2蛋白 (p0．05)。10只正常对照组和10只假手术组沙鼠神经元和神经胶质 细胞内均有Smad2和Smad4蛋白阳性表达，但两组结果没有显著性 差异(pO，05)。同正常对照组比较，脑缺血组20只沙鼠脑神经元和 神经胶质细胞在缺血再灌注6小时、l天时Smad2和Smad4蛋白表 达显著上调(PO．05)。同脑缺血组相比，西比灵干预组20只沙鼠神 经元和神经胶质细胞内Smad2和Smad4蛋自在缺血再灌注6小时、 1天时表达明显下调fP0．05)。 结论： 1．正常沙鼠脑神经细胞和神经胶质细胞内均有Smad2和Smad4 蛋白表达。 2，沙鼠脑缺血再灌注6小时、1天后，脑神经细胞和神经胶质细 胞内Smad2和Smad4蛋白表达上调；西比灵干预后，缺血再灌注沙 鼠脑神经细胞和神经胶质细胞内Smad2和Smad4蛋白表达下调。提 示Smad2和Smad4蛋白可能是缺血性脑损伤程度的又一重要标志。 3．Smad4和Smad2蛋自在正常沙鼠脑组织内及脑缺血的发生和 发展过程中可能有协同作用。 关键词：Smad蛋白，免疫组化，缺血再灌注，西比灵，沙鼠 堡兰堂垡鱼圣 堡兰堂垡鱼圣 墓塞摘要 ABSTRACT Objective：To study the expressions of Smad2 and Smad4 protein and the effects of flunarizine on the expressions of Smad2 and Smad4 protein in brain tissue following cerebral ischemic reperfusion in gerbils． Methods：A cerebral ischemia．reperfusion model in gerbils was established by clamping both common carotid．One group of gerbils were fed with flunarizine before experiment．The expressions of Smad2 and Smad4 protein in brain tissue were detected by using immunohisto— chemistry technique at the 6th hour、1 st day after ischemic reperfusion (m)．Experimental results were comparatively analysed with statistic methods ofSpssll．O． Results：Experimental results revealed that Smad2 and Smad4 protein were expressed in neurons and gliacytes of brain tissue in 60 gerbils，and expression of Smad4 protein is higher than that of Smad2 protein(p0．05)．Smad2 and Smad4 protein were present in neurons and gliacytes both normal contral group of 10 gerbils and sham operation group of 1 0 gerbils，and there was no significant difference between two groups(尸0．05)．Compared with normal control group，the expression of Smad2 and Smad4 protein in neurons and gliacytes of ischemic gerbils was evidently increased at the 6th hour、1 st day(pO．05)．Compared with cerebr