白藜芦醇对重症急性胰腺炎大鼠肝脏的保护作用.doc

作者：沙焕臣，马清涌，JHA Rajiv Kumar，徐复国，马振华 作者单位：西安交通大学医学院第一附属医院肝胆外科，陕西西安 710061 【摘要】 目的 探讨白藜芦醇对实验性重症急性胰腺炎(severe acute pancreatitis, SAP)大鼠肝功能的保护作用。方法 96只成年雄性SD大鼠随机分为假手术(sham operation, SO)组、重症急性胰腺炎(SAP)组、地塞米松(dexamethasone, DEX)治疗组和白藜芦醇(resveratrol, RES)治疗组。各组大鼠均于制模后3、6、12h采集标本。生化方法进行肝功能测定;酶联免疫吸附试验(enzyme linked immunosorbent assay, ELISA)测定血清TNF 和IL6含量;透射电镜观察胰腺和肝脏超微结构的变化;RTPCR检测凋亡调控基因Bax、Bcl2和caspase3 mRNA表达;Western Blot检测凋亡调控基因Bax、Bcl2、caspase3和线粒体内细胞色素C蛋白的表达。结果 SAP组各时间点血清谷丙转氨酶(alanine aminotransferase, ALT)、谷草转氨酶(aspartate aminotransferase, AST)和总胆红素(total bilirubin, TBIL)水平均明显高于SO组(P 0.05)，RES组和DEX组肝功损害较SAP组明显降低(P 0.05)。RES组和DEX组TNF 、IL6含量均明显低于SAP组(P 0.05)。SAP组出现明显细胞肿胀、毛细血管淤血、血栓形成、线粒体肿胀和细胞凋亡等超微结构的变化，RES组和DEX组上述改变明显减轻。RES组Bax、caspase3 mRNA表达显著低于SAP组(P 0.05)，Bcl2 mRNA表达明显高于SAP组。Western blot 检测结果与RTPCR结果一致。RES组各项指标与DEX组差异不显著(P 0.05)。结论 白藜芦醇可以降低SAP大鼠肝细胞凋亡的发生，从而起到减轻SAP介导的肝脏功能损伤的作用。 【关键词】 重症急性胰腺炎;白藜芦醇;肝功能损害;Bax;Bcl2;caspase3;细胞色素C in rats with severe acute pancreatitisSHA Huanchen, MA Qingyong, JHA Rajiv Kumar, XU Fuguo, MA Zhenhua (Department of Hepatobiliary Surgery, the First Affiliated Hospital, Medical School of Xian Jiaotong University, Xian 710061, China)ABSTRACT: Objective To demonstrate the protective effect of resveratrol (RES) on the hepatic function in rats with severe acute pancreatitis (SAP). Methods Ninetysix male SpragueDawley (SD) rats were randomly divided into four groups: shamoperation (SO) group, SAP group, dexamethasonetreated (DEX) group and resveratroltreated (RES) group, each group having 24 rats which were evaluated at 3, 6, and 12h during the experiment. Serum hepatic function was determined by biochemical detection. Serum TNF and IL6 levels were determined by ELISA. The ultrastructure of hepatic and pancreatic tissues was observed by transmission electron microscope. The expressions of the apoptosisrelated genes and proteins Bcl2, Bax caspase3 and cytochrome C were observed using semiquantitative RTPCR and We