高效液相色谱离子阱飞行时间质谱法鉴定碱性嫩黄中.PDF

高效液相色谱离子阱飞行时间质谱法鉴定碱性嫩黄中.PDF

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2019年3月 Vol.37 No.3 March 2019 ChineseJournal of Chromatography 299~304 Special issue on celebration of International Working Women’s Day (2019)by female chromatographicscientists ·Article DOI:10.3724/ SP.J.1123.2018.10025 Identification of the impurity in auramine O by high performance liquid chromatography⁃ion trap⁃time of flight mass spectrometry and preparation of the auramine O reference standard by preparative high performance liquid chromatography 1 1 2 2 1∗ CHE Fenfang ,LU Yanzhen ,XI Xingjun ,LAN Tao ,WEI Yun (1.StateKeyLaboratory of ChemicalResourceEngineering,Beijing University of Chemical Technology, Beijing 100029,China;2. ChinaNationalInstitute of Standardization,Beijing 100191,China) Abstract:A novel and effectivemethodwas establishedforthequalitativeanalysis of impuritiesin auramine O samples of illegal food additives using high performance liquid chromatography⁃ion trap⁃time of flight mass spectrometry (HPLC⁃IT⁃TOF⁃MS). An impurity was identified in the auramine O sample using the optimized HPLC⁃IT⁃TOF⁃MSmethod. According tothe exact mass of each fragment ion measured by multistage MS,the structure of the impuritywasdeterminedtobethat of4⁃(imino (4⁃(methylamino)phenyl)methyl)⁃N,N⁃dime⁃ thylaniline hydrochloride. The synthetic route of the auramine O andthe source of the identified impurity were proposed. Simultaneously,apreparative high performance liquid chromatography (prep⁃HPLC) technique was successfully appliedforthepurificationoftheauramineOfromcomplexsamples.Prep⁃HPLCcolumnswithp

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