Comparative analysis of sequence feature and expression of two heat shock cognate 70 genes in mandarin fish Siniperca chuatsi 川芎两个热激同源70基因序列特征及表达的比较分析.pdf

Gene 560 (2015) 226–236 Contents lists available at ScienceDirect Gene journal homepage: www. /locate/gene Comparative analysis of sequence feature and expression of two heat shock cognate 70 genes in mandarin fish Siniperca chuatsi Pengfei Wang a,b, Peng Xu a, Shuang Zeng a, Lei Zhou a, Lei Zeng a, Guifeng Li a,⁎ a Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China b South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China a r t i c l e i n f o a b s t r a c t Article history: Heat shock cognate protein 70 (HSC70) is a molecular chaperone that plays essential roles in maintaining the cel- Received 23 September 2014 lular protein homeostasis. In this study, two HSC70 isoforms were identified and characterized from mandarin Received in revised form 28 January 2015 fish Siniperca chuatsi. They have similar sequence structures, containing seven introns in their coding regions Accepted 5 February 2015 and sharing 94% similarity of their deduced amino acid sequences with 38 substitutions. Transcripts of both iso- Available online 8 February 2015 forms were detected throughout the embryogenesis, at low levels during the early developmental stages and up- Keywords: regulated at blastula for ScHSC70-1 and appearan