研究生干细胞在血液系统疾病中的研究应用.ppt

* * These are some of my results.First pictures shows Kaumi-1,which is a AML M2 type cell line ,with t(8,21)character, * * This slide are,,,,,,,,.left side is Kasumi-1 cells.Right side is nomal bone marrow mononuclear cells. The target cells were CFSE positive and effect cells were CFSE negative. 7-AAD stained dead cells.Then we caculated the percentage of 7-AAD high in CFSE high population. * * Then we increased the time of incubation to 8hs,and we observed that nomal bone marrow mononuclear cells were still not killed,while there was an increasing cytotoxicity of r9s2 cells to some of the cell lines. . * * These are confocal microscopy pictures. we chose the most sensitive cell line Kasumi-1 as target cells.The green cells are CFSE labled r9s2 T cells and the red cells are DII labled Kasumi-1 cells.We observed Vγ9Vδ2 T lymphocytes captured the Kasumi-1 cells’ membrane fragments after 4h coincubation, as the edge of r9s2T cells has a yellow fusion signal. * * This slide is another confocal microscopy result. The red cells are DII labeled Kasumi-1 cells,green cells are CFSE labled rs T cells.Blue ones are both nuclears labled with Hochest 33258..And these are merge pictures.. We noted the membrane of Kasumi-1 brake,and the nuclear became inregularly afrer 4 hours killed by r9s2 T cells. From these two slides,we concludeed Vγ9Vδ2 T lymphocytes could bind and trogocytose AML cell lines via capture of AMLcells membrane fragments and induced cell daath. 红色荧光DiI染Kasumi-1细胞膜，绿色荧光CFSE染Vγ9Vδ2 T 细胞骨架，Hochest33258染两者细胞核 正常时， Kasumi-1细胞膜完整，核饱满 两者作用8h后，观察到Kasumi-1细胞膜崩解，荧光渗漏，核成不规则多行性 * Figure 1 | The multipotentiality of MSCs. This figure shows the ability of mesenchymal stem cells (MSCs) in the bone-marrow cavity to self-renew (curved arrow) and to differentiate (straight, solid arrows) towards the mesodermal lineage. The reported ability to transdifferentiate into cells of other lineages (ectoderm and endoderm) is shown by dashed arrows, as transdifferentiation