黄孢原毛平革菌酵母双杂交cDNA文库的构建及应用.pdf

　2006, 12(5):678～682 　 　 Ch in J A pp lE n viron B io l=ISSN 1006-68 7X 　 2006-10-25 　 cDNA* ** 胡国库　颜永红　姜 权　吴波　冯 红　张义正 ( /　　610064) 　　, -. 5 5 2 d 3 d cDNA, 2. 5×10 3. 0×10 . 14-3-3 3 d cDNA, SD -/Ade-/ H is-/ Leu-/ Trp 600 , , 30 3. DNA . HaeⅢSau3AⅠ 4. , 14-3-3 , 14-3-3, 3 GenBan . SBASE , Y2H333 OB-fold(OB-fold nucleic acid binding dom ain). , cDNA , 14-3-3 -. 4 1 28 　;;cDNA ;14-3-3 CLC　Q785 Construction and Application ofY east Two Hybrid cDNA L ibraries * ofPhanerochaete ch rysosp orium ** HU Guo u, YAN Yonghong, JIANG Quan, WU Bo, FENG HongZHANG Yizheng (Colleg e of Lif eSciences, Sichuan University, S ichuan Key Laboratory of M olecular B iology and B iotechnology, Chengdu 610064, China) Abstract　There has been no report on protein - protein interaction inPhanerochaete chry sosporium, amodelm icroorgan ism for 5 5 studying lignin degradation. In th is study, wt o yeast wt o-hybrid libraries containing 2. 5×10 and 3. 0×10 recomb inan ts, were constructed using 2 d and 3 d mRNAs derived from P. chry sosporium cultures grow ing in low-nitrogen m edium. 14-3-3 gene w as used to screen the 3-day library by yeast two-hybrid technique and m ore than 600 clonesw e