固定化蛋白质在沸石和介孔分子筛上的直接电子转移及生物传感.pdf

34 2 () Vol. 34 No. 2 2011 6 JOURNAL OF NANJING NORMAL UNIVERSITY ( Natural Science E ition) June, 2011 , (, 210046) [ ] . . , , . , . [ ] , , , , [ ] O646. 54 [] A [ ] 1001-4616( 2011) 02-0054-06 Direct Electron Transfer of Proteins Immobilized on Zeolite/MesoporousMolecular Sieves and Their BiosensingApplications iu Qian, DaiZhihui ( School of Chem istry an Environm ental Science, Nanjing N orm alUniversity, N anjing 210046, China) Abstract: Direct electron transfer between re ox proteins an electro es is of practical an theoretical interest an can be mi prove by electro e or protein m o ification. The irect contact of protein w ith electro e surfaces can lea to a sig- n ifican t change of the protein structure an /or function. Immob ilize m icroporous an m esoporous m olecular sieves on electro e surfaces provi es am icroenvironm ent smi ilar to that of the re ox protein in native system s an gives the protein m olecu lesm ore free om in orientation, thus facilitating the irect electron transfer through the con ucting tunnels ofm o- lecu lar s ieves. Th is brief review focuses on the m icroporous an m esoporous m olecu lar sieves use for protein mi m obi- lization, electron transfer an b iosensing, w ith emphasis on recent a vances, challenges an tren s. K ey words: protein, zeolite, m esoporous sieves, irect electron transfer, biosensor , . , , . , , [ 1] . , . [ 2] . [ 3] , , , , . , . , . , .