槐米中芦丁的提取及分离工艺研究.doc

槐米中芦丁的提取分离工艺研究 2014届本科生毕业论文 PAGE 10 PAGE 12 学号 2010261130 毕 业 论 文（设 计） （ 2014 届本科） 论文题目: 槐米中芦丁的提取及分离工艺研究 学 院： 陇东学院化学化工学院 专 业： 应用化学 班 级： 10应用化学 作者姓名： 指导教师： 职称： 教授 完成日期： 2014 年 4 月 20 日 槐米中芦丁的提取及分离工艺研究 摘 要：目的 以槐米为原料提取黄酮类化合物芦丁，学习黄酮类化合物的提取、分离、鉴定的原理和方法，掌握黄酮类成分的主要性质，优选出提取芦丁的工艺条件。方法 主要采用碱提酸沉法提取分离芦丁，再由芦丁在稀酸中水解得到槲皮素，用单因素法优选出提取的最佳条件。结果 用碱提酸沉法提取的芦丁质量合格，且颗粒大小、提取次数、提取时间、提取温度、酸度、结晶时间等因素均对芦丁的产率和纯度有影响。结论 此方法装置简单，操作方便，费用和成本低，但提取过程中很难过滤，效率不是很高。各提取因素中酸度对芦丁的产率影响最大。 关键词：槐米；芦丁；槲皮素；碱提取酸沉淀；鉴定 Study on Extraction and Separation of Rutin in Flos Sophorae Zhenzhu Li (College of Chemistry and Chemical Engineering, Longdong University Abstract: Objective To extract Rutin from Sophora flavone compounds, learning flavonoids extraction, separation, identification principle and method, grasping the main properties flavonoids, and choosing the optimum extraction technology of rutin. Methods Mainly using the method of alkali extraction and acid precipitation to extract rutin, the next, making quercetin by hydrolyzing rutin in dilute acid, selecting the best extraction conditions by single factor method. Result The quality of rutin that made by alkali extraction and acid precipitation method is eligible, and particle size, extraction times, extraction time, extraction temperature, acidity, crystallization time and other factors have effect on the yield and purity of rutin. Conclusion This method has the advantages of simple device, convenient operation, low charge and cost, but the extraction process is difficult to filter, the efficiency is not high. In the factors of extracting rutin, acidity is the greatest impact to the yield. Keywords: Sophora japonica; rutin; quercetin; alkali extraction and acid precipitation; identification 0 引 言 槐米，别名白槐、槐花，为豆科植物槐（Sophora japonica L.）的未开放花蕾，两千年前即作药用，公元2800多年前的《神农本草经》首次将槐米列为上品[1]。《中国药典》称其凉血止血、清肝泻火，用于便血、痔血血