响应面法优化脐橙渣中水溶性膳食纤维提取工艺.PDF

※工艺技术 食品科学 2012, Vol. 33, No. 04 109 吴笑臣，王科军，钟金莲，罗序中 * (赣南师范学院化学与生命科学学院，江西 赣州 341000) 以赣南脐橙渣为原料，采用纤维素酶制备水溶性膳食纤维。在单因素试验基础上，选取酶解温度、加 酶量(质量分数)和酶解时间为响应变量，以水溶性膳食纤维得率为响应值，利用 Box-Behnken 试验设计方案和响应 面分析法，建立水溶性膳食纤维得率与响应变量的回归方程，并确定最佳提取条件为酶解温度 4 8 ℃、加酶量 1.25%、酶解时间5h ，此条件下水溶性膳食纤维得率为 13.11%，与预测值 13.14% 较为一致。 脐橙渣；水溶性膳食纤维；响应面分析法；优化 Response Surface Methodology for Optimization of Extraction Process of Soluble Dietary Fiber from Navel Orange Residue WU Xiao-chen ，WANG Ke-jun ，ZHONG Jin-lian ，LUO Xu-zhong* (College of Chemistry and Life Science, Gannan Normal University, Ganzhou 341000, China) Abstract ：Navel orange residue was treated with cellulase to extract soluble dietary fiber. Extraction parameters including extraction temperature, cellulase dose and length of extraction time were optimized using Box-Behnken design and response surface methodology based on single factor investigations for achieving maximum yield of soluble dietary fiber. A regression equation reflecting the relationship between the yield of soluble dietary fiber and the extraction parameters was set up. The optimal extraction parameters were found as follows: extraction temperature, 48 ℃; cellulase dose, 1.25%; length of extraction time, 5 h. Under the optimized conditions, the actual yield of soluble dietary fiber was 13.11%, close to the predicted value of 13.14%. Key words ：navel orange residue ；soluble dietary fiber ；response surface methodology ；optimization 中图分类号：TS201.2 文献标识码：A 文章编号：1002-6630(2012)04-0109-06 膳食纤维(dietary fiber ，DF) 具有预防肥胖症、缓 等许多方面比水不溶性膳食纤维(insoluble dietary fiber ， 解便秘、改善肠道菌群、降低血糖、降低血清胆固醇 IDF)具有更强的生理功能而成为研究报道的热点之一[8-10] 。