体细胞核移植后核重编程的影响因素-生命科学.PDF

18 4 Vol. 18, No. 4 2006 8 Chinese Bulletin of Life Sciences Aug., 2006 1004-0374(2006)04-0355-06 200025 Q343.5; Q813A Influencing factors of nuclear reprogramming after somatic cell nuclear transfer LI Yan, FENG Yun*, SUN Yi-Juan (Reproductive Center, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China) Abstract: In recent years, human somatic cell nuclear transfer-embryo stem (SCNT-ES) research has become more and more influential and powerful. It is the regeneration medicine which gives the new idea to degenerative disease and organ transplantation. However, nuclear reprogramming, which stands in the way of reconstructed embryo stem cell research, is still a bottleneck in the procedure of nuclear transfer. Nuclear reprogramming means that the donor nuclei must erase the previous memory of cell differentiation and go back to a totipotent status. It has a pridominant effect on reconstructed embryo development. There are many influencing factors of nuclear reprogramming, such as the age of somatic cell donor, the source of somatic cell and its differentiation,cycle stage, passage number