本科毕业论文动物科学专业.docVIP

本科毕业论文 题 目 非抗性筛选生长抑素基因疫苗对育肥猪的 免疫应答研究 姓 名 学 号 专 业 动物科学 指导教师 职 称 中国·武汉 目 录 TOC \o 1-3 \h \z \u 摘 要 = 1 \* ROMAN I 关键词 = 1 \* ROMAN I Abstract = 1 \* ROMAN I Keywords = 1 \* ROMAN I 前言 1 1 材料与方法 2 1.1 材料 2 1.1.1 质粒和菌株 2 1.1.2 实验试剂 2 1.1.3 实验仪器 2 1.1.4 实验动物 2 1.2 疫苗准备和免疫方法 2 1.2.1 疫苗准备 2 1.2.2 免疫方法 2 1.3血样制备 3 1.4 抗生长抑素抗体检测 3 2 结果与分析 3 2.1非抗性筛选生长抑素免疫育肥猪后试验组与对照组抗体水平比较 3 2.2 不同剂量的PGS/2SS–asd质粒免疫各组育肥猪抗体水平 4 3 讨论 4 3.1非抗性筛选生长抑素试验组与对照组之间的抗体效价比较。 4 3.2免疫剂量对免疫效果的影响 5 4 小结 5 参考文献 6 致 谢 7 摘 要 将90只60日龄育肥猪均分为6组，T1-T4组用非抗性筛选生长抑素基因疫苗pGS/2SS-asd免疫，剂量浓度分别是2×108 CFU/mL，2×109 CFU/mL，2×1010 CFU/mL，2×1011 CFU/mL；C1和C2组为对照组，分别免疫C500空菌和PBS。整个试验共免疫三次，每次免疫间隔4周。ELISA抗体检测结果显示：试验组在免疫初期抗体水平高于对照组，但是免疫后期差异不明显，不同免疫剂量组之间的抗体水平差异不明显。总之，用新型生长抑素基因疫苗免疫育肥猪在免疫初期可以检测到高水平的抗生长抑素抗体，推测出此DNA疫苗免疫大动物可以很好地促进动物的生长。 关键词 pGS/2SS-asd；生长抑素；减毒沙门氏菌；基因疫苗；育肥猪 Abstract A total of 90 commercial pigs at the age of 60 days were divided into 6 groups. Animals in GroupC2 were orally administered with PBS as a black control,What were administered GroupC1 is C500-salmonella.GroupT1 to GroupT4 were immunized with pGS/2SS-asd ,the density of dos of GroupT1 is 2×108 CFU/mL,GroupT2 is 2×109 CFU/mL，GroupT3 is 2×1010 CFU/mL and GroupT4 is 2×1011 CFU/mL；Three times immunizing,the interval of immunizing toties quoties is 4 weeks.The results of ELISA antibody detection show that:The test Group have a higher level of anitiboty in the initial stage of immunization. But the difference with them were not obviously in the later stage.the difference of the level of anitibody that from GroupT1 to GroupT4 is not obviously too.In all, use the gene vaccine of pGS/2SS-asd can get high abtibody of the SS from the analyze of the ELISA results. Conclude that this kind of DNA vaccine can accelerate the growth rat