细胞生物学会干细胞教学课件.pptx

大脑皮层发育中神经前体细胞的命运决定Cell fate Determination of Neural Progenitor Cells in Cortical Development of Cerebrum;The Outline;Cortex: the frontal, dorsal and outer part of the brain;DeFelipe, 2011; bar: 10cm;?Santiago Ramón y Cajal;?Santiago Ramón y Cajal;Woodworth Macklis, 2012;Key Questions Regarding Cortical Development;The cerebral cortex (neocortex) of the brain The inside-out pattern of cortical neurogenesis Neural progenitor cells (NPCs) in cortical neurogenesis and gliogenesis Cell fate determination of cortical NPCs: progressive fate restriction vs. NPC heterogeneity Conclusion and future direction ;Two Major Types of Cortical Neural Progenitor Cells;Indirect Cortical Neurogenesis;;The cerebral cortex (neocortex) of the brain The inside-out pattern of cortical neurogenesis Neural progenitor cells (NPCs) in cortical neurogenesis and gliogenesis Cell fate determination of cortical NPCs: progressive fate restriction vs. NPC heterogeneity Conclusion and future direction ;IV. Cell fate determination of NPCs: Progressive fate restriction vs. NPC heterogeneity;Transplantation Experiments Using 3H-Thymindine labeled Cortical NPCs Indicates Progressive Fate Restriction;Ferret Layer 4 Cortical NPCs Can Generate Layer 2/3 Neurons (Late-born Neurons);Ferret Layer 4 Cortical NPCs are Unable To Generate Layer 6 Neurons (Early-born Neurons);;;;Debates Aroused Again! Upper-layer Specific NPCs?;Franco Muller, 2012;;Genetically Labeling Individual Cortical NPC Clones Using Mosaic Analysis With Double Markers (MADM);Progressive Fate Restriction of Individual Radial Glial NPCs in Producing Excitatory Neurons;One in Six Radial Glial NPCs Proceeded to Gliogenesis at the End of Neurogenesis ;V. Conclusion and Future Direction;;;Scramble;Thank you!