单细胞测序及其免疫学应用.ppt

单细胞测序及其免疫学应用 Rationale：Why should we do it? Content: What will we do? Impact: If we did it, so what? Challenge: What is the limitation? Perspective: How could we do better? 2014学年第一学期《实验生物学》课程 LET’S Discuss it after the course break. 单细胞测序及其免疫学应用 Rationale：Why should we do it? Content: What will we do? WHAT’s the Impact of Single Cell Seq? Perspective: How could we do better? 2014学年第一学期《实验生物学》课程 OPEN DISCUSSION: 单细胞测序及其免疫学应用 Rationale：Why should we do it? Content: What will we do? Impact: If we did it, so what? Challenge: What is the limitation? Perspective: How could we do better? 2014学年第一学期《实验生物学》课程 THINK IT HARD and get your own answer! 单细胞测序及其免疫学应用 Rationale：Why should we do it? Content: What will we do? Impact: If we did it, so what? Challenge: What is the limitation? Perspective: How could we do better? 2014学年第一学期《实验生物学》课程 What is the limitation? 2014学年第一学期《实验生物学》课程 Adapted from Jaena Han 单细胞测序及其免疫学应用 Rationale：Why should we do it? Content: What will we do? Impact: If we did it, so what? Challenge: What is the limitation? Perspective: How could we do better? 2014学年第一学期《实验生物学》课程 Workflow for amplifying and sequencing cellular DNA and RNA. /nmeth/journal/v11/n1/images/nmeth.2771-I1.jpg Workflow for amplifying and sequencing cellular DNA and RNA. /nmeth/journal/v11/n1/images/nmeth.2771-I1.jpg WHAT IF you want to detect both DNA and RNA in one SINGLE Cell? Microfluidic device designed for single-cell mRNA/gDNA separation and whole pool amplification /pmc/articles/PMC4175731/ Content: What will we do? SINGLE cell Genomics seq SINGLE cell Transcriptomics seq SINGLE cell Proteomics seq SINGLE cell Metabolimcs seq …… 2014学年第一学期《实验生物学》课程 SINGLE cell Proteomics seq 2014学年第一学期《实验生物学》课程 HOW? 单细胞测序及其免疫学应用 2014学年第一学期《实验生物学》课程 Cxcl1 gene expression in a “homogeneous” group of dendritic cells (DC). Image courtesy of Alex Shalek From The Broad Institute, MIT Aviv Regev@MIT 单细胞测序及其免疫学应用 2014学年第一学期《实验生物学》课程 Aviv Regev@MIT 单细胞测序及其免疫学应用 Alex K. Sha