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Community structure and dynamics of bacteria in a
water recirculating system for culturing european eel
Abstract
Recirculating aquaculture system (RAS) is typically an indoor system that allows farmers to
control environmental conditions year round. It has many advantages, such as recirculating
aquaculture water use, decreasing water consumption, reducing disease risk, high efficiency
untilization of resource and little pollution to environments and so on. RAS enable the
aquaculture produce year round without regional or climatic restrictions, it is an important way
for the sustainable development of aquaculture. Degrading organics in aquaculture water is the
key link in RSA. Bacteria are efficient decomposers for organics in aquaculture environments.
Bacteria have great diversity of species and metabolic pathways, they are important regulators
for environmental conditions and play important roles in materials cycling and energy flowing.
The aim of this work is to explore the community structure and dynamics of bacteria in a water
recycling system culturing European eel. It is hoped that the information generated in this study
can be served as theoretical guidance for verifying the effect of recirculating aquaculture system,
improving the design of the system and facilitating the application of the system.
Water samples were collected from each treatment of the recirculating system on April 15,
July 15, September 15 and December 15, 2009. Attached samples were collected from biofilms
on the nylon net and the oysters shells. The quantity and the composition of bacteria were
determined by plate count method and 16S rRNA gene clone library analysis.
1) Variations of bacterial quanity in water of the recirculating aquaculture system during
four
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