中英文翻译对照.doc

PARAOXONASE-1 ACTIVITY IN SUBFERTILE （低生育力）MEN AND RELATIONSHIP TO SPERM PARAMETERS2008 对氧磷酶1活性在低生育力男性及精子参数的关系 该文提到特发性不育男性与精液异常低生育男性比较，前者显示高ROS水平，PON-1活性改变无统计学意义，后者PON-1活性降低有统计学意义 Abstract Oxidative stress has been implicated in the pathogenesis of male infertility. Paraoxonase-1 (PON-1) is a high density lipoprotein associated antioxidant enzyme that prevents oxidative modification of low density lipoprotein. Our aims of the study were to investigate i) Seminal PON-1 activity in subfertile men ii) whether it had any relationship with semen parameters. The study included 28 idiopathic subfertile, 32 subfertile male with abnormal semen parameters and 30 fertile volunteers. Seminal PON-1 activity was measured spectrophotometrically. Seminal total antioxidant status (TAS) and total oxidant status (TOS) were determined by using colorimetric methods. Oxidative stress index (OSI) was calculated as [(TOS/TAS) ×100]. TOS and OSI were significantly higher and PON-1 activity and TAS were significantly lower in subfertile men than in idiopathic subfertile men and fertile donors. PON-1 activity was also strongly correlated with sperm concentration (r=0.68, p0.0001), motility (r=0.58, p0.0001) and morphology (r=0.62, p0.0001) in overall group. The receiver operating characteristic curve (ROC) analysis revealed a high diagnostic value for PON-1 activity with respect to male factor subfertility, with an area under curve of 0.95 (95% confidence interval (CI) =0.89-1.01), sensitivity = 97 % and specificity = 88%. Men with abnormal semen parameters have decreased levels of PON-1 activity in their seminal plasma. This may play an important role in the pathogenesis of male factor subfertility. 摘要在男性不育症的发病机制，氧化应激有牵连。对氧磷酶-1（PON- 1）是一个高密度脂蛋白相关的抗氧化酶，防止低密度脂蛋白氧化修饰。我们的研究目的是调查I）精液的PON-1活性低生育力男性二）是否有任何与精液参数的关系。这项研究包括28例特发性低生育力，30例精液参数异常的低生育力男性和32例肥沃志愿者。精液PON- 1活性的测定分光光度法。精浆总抗氧化状态（TAS）和总氧化剂状态（TOS）利用比色法测定。氧化应激指数（OSI）的计算公式为[（TOS/ TAS）× 100]。 TOS和OSI显著较高的PON- 1活性和TAS显著低于低生育力男性特发性低生育力的男性和肥沃的捐助者。 P