Rapid diagnosis of Mycoplasma pneumoniae in children with pneumonia by an immunochromatographic antigen assay 免疫层析法快速诊断小儿肺炎支原体.pdf

/scientificreports OPEN Rapid diagnosis of Mycoplasma pneumoniae in children with pneumonia by an immuno- Received: 21 April 2015 Accepted: 28 September 2015 chromatographic antigen assay Published: 21 October 2015 1 1 1 1 2 1 Wei Li , Yujie Liu , Yun Zhao , Ran Tao , Yonggang Li Shiqiang Shang Mycoplasma pneumoniae is a particularly important pathogen that causes community acquired pneumonia in children. In this study, a rapid test was developed to diagnose M. pneumoniae by using a colloidal gold-based immuno-chromatographic assay which targets a region of the P1 gene. 302 specimens were analyzed by the colloidal gold assay in parallel with real-time PCR. Interestingly, the colloidal gold assay allowed M. pneumoniae identification, with a detection limit of 1 ×103 copies/ml. 76 samples were found to be positive in both real-time PCR and the colloidal gold assay; two specimens positive in real-time PCR were negative in the rapid colloidal gold assay. The specificity and sensitivity of the colloidal gold assay were 100% and 97.4%, respectively. These findings indicate that the newly developed immuno-chromatographic antigen assay is a rapid, sensitive and specific method for identifying M. pneumoniae, with potential clinical application in the