基于右旋糖酐蔗糖酶转糖基作用的槲皮素葡萄糖苷的合成研究.docx

doi∶10． 3969 / j. is doi∶10． 3969 / j. issn. 2095 － 1736． 2014. 05. 093 基于右旋糖酐蔗糖酶转糖基作用的 槲皮素葡萄糖苷的合成研究 亮，张洪斌，胡雪芹，牛博艺 任 (合肥工业大学 制药工程系，合肥 230009) 摘 要 右旋糖酐蔗糖酶是一种以蔗糖为唯一底物，将蔗糖分子中 D － 葡萄糖基催化转移到受体分子上的葡萄糖 基转移酶。利用右旋糖酐蔗糖酶的转糖基作用，以蔗糖为葡萄糖糖基供体，槲皮素为糖基受体，对槲皮素糖苷的酶 法合成进行了探索。通过对该酶催化反应体系、催化反应条件及产物分析的研究，结果表明:在 25° C 下，右旋糖酐 蔗糖酶能够在 30% DMSO － 70% 乙酸 － 乙酸钙(0. 02 mol / L，pH 值 5. 4 ) 的反应体系中催化合成一种槲皮素葡萄糖 苷，在这个反应体系下，以 10% 的蔗糖作为糖基供体，槲皮素为糖基受体，右旋糖酐蔗糖酶活力为 40 U / mL，转速为 150 r / min，槲皮素糖苷的转化率最高，可达 39. 5% 。通过质谱分析确定是一种槲皮素单糖苷，分子量为 464。该研 究结果为黄酮类物质的糖基化修饰奠定了基础。 关键词 右旋糖酐蔗糖酶;转糖基;槲皮素;槲皮素葡萄糖苷 文章编号 2095 － 1736(2014)05 － 0093 － 06 中图分类号 Q814． 2 文献标识码 B Study on synthesis of quercetin glucoside based on transglycosylation of dextransucrase ＲEN Liang，ZHANG Hong-bin，HU Xue-qin，NIU Bo-yi (Department of Pharmaceutical Engineering，Hefei University of Technology，Hefei 230009，China) Abstract Dextransucrase is a glucosyltransferases catalyzed by transferring D-glucopyranosyl units in sucrose to the receptor molecule to synthesize a-glucan or oligosaccharides． This paper explored the synthesis of quercetin glycoside by using sucrose as glucose glycosyl donor，quercetin as glycosyl receptor and applying the transglycosylation of dextransucrase． Enzymatic glucosylation in aqueous-organic solvents，the conditions of catalytic reaction and the structure of the product were also studied． The result showed that a new quercetin glycoside was achieved after reaction catalyzed by dextransucrase in a mixture of DMSO(30% ) / Acetic acid calcium acetate buffer (70% )(0. 02 mol / L，pH = 5. 4)at the 25℃ ． Under this reaction system，using 10% of sucrose as glucose glycosyl donor，quercetin as glucose glycosyl receptor and 40 U / mL dextransucrase，the stirring speed at 150 r / min，the conversion of quercetin glycoside could be high，up to 39. 5% ． The product was characterized by MS and confirmed as a quercetin mono-glycoside，and the molecular weight of the product is 464． The results of this study lays a foundatio