NF2κB信号通路在食管鳞癌细胞系中的激活.pdfVIP

肿瘤防治研究 2006 年第 33 卷第 1 期 · 11 · κ N F B 信号通路在食管鳞癌细胞系中的激活 1 1 1 1 2 1 田　芳 ,许培荣 ,侯卫红 ,刘红涛 ,陈奎生 ,薛乐勋 κ The Activation of NF B Signal ing Transduction Pathway in Esophageal Squamous Cell Carcinoma Cell L ines TIAN Fang1 ,XU Peirong1 , HOU Weihong1 , L IU Hongt ao 1 ,C H EN Kuisheng2 , XU E L exun1 1. L aboratory f or Cel l B iology , Z hengz hou Uni ve rs i ty , Z heng z hou 450052 , Chi na; 2 . D ep a rtment of p at hology , The F i rs t A f f i l i ate d H osp i t al , Z hengz hou Uni vers i ty Corresp on d i ng A ut hor : X UE L ex un , Em ai l : X uelx @37 1. net Abstract :Objective 　More and more evidences have shown t hat t ran scrip tion factor N uclear Factorkap ( κ) p aB N F B play s a critical role in t he initiation and p rogression of carcino genesi s. In t he p resent st udy , κ we investigat ed whet her t he N F B signaling t ran sduction p at hway wa s con stit utively activat ed in ESCC cell lines. Methods 　Immunocytochemi st ry and West ern blotting were u sed to det ermine t he p rot ein level s κ κα β of t he subunit s of N F B ,p 50 and p 65 , inhibitor B and I KK in two ESCC cell lines. N uclear p rot ein s f rom t he ESCC cell lines were ext ract ed to evaluat e t he DN Abinding activit y by elect rop horetic mobilit y gel shift assay ( EM SA) . Results 　Immunocytochemical analysi s showed t hat p 50 and p 65 , two rep resent κ κα κ β ative subunit s of N F B , I B , a nat ural inhibitor of N F B , and it s up st ream kina se I KK were mainly exp ressed and localized in t he cytoplasm . Cytop la sm p rot ein ext ract s f rom t he cell lines each were ana