分子细胞生物学前沿博士.pptxVIP

分子细胞生物学专题----转录延伸因子P-TEFb及其活性调控;Main Points;I. Transcription process;Steps of class II gene transcription;TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH RNA polymerase II (Pol II);RNA polymerase II (Pol II);Pre-initiation;PIC;Pol II;Pol II;Early elongation;Splicing;;Steps: Pre-initiation Initiation Promoter clearance Elongation Termination ;P-TEFb, its functions and activity regulation;P-TEFb: composition and property ;Physiological functions;;B. Pathological functions;;Trans-cyclin T1 gene causes cardiac hypertrophy;CycT1;;;;CycT1;CycT1;How does cell signaling control the disruption of the inactive complex?;Control P-TEFb activity by calcium signaling pathway;UV and HMBA can trigger Ca+2 influx;;Calcium signaling pathways;;Calcium signaling pathways;;Hiding and seeking ….;? isoform of PP1 catalytic subunit involves in mediating the disruption of 7SK snRNP;;;;;;How do PP2B and PP1 cooperatively disrupt inactive P-TFb complex?;;;CycT1;CycT1;How is liberated P-TFFb Recruited to chromatin;;;Modified Nuclear Fractionation;Almost all of Brd4 associate with chromatin at basal state;Stimuation induces Brd4 release from chromatin;The release of Brd4 from chromatin is essential from P-TEFb recruitment;The release of Brd4 from chromatin is HDAC-dependent;The release of Brd4 from chromatin is essential from elongation;CycT1;HDAC;Thank you!