NGS与高通量测序技术课件.ppt

https://labiotech.eu/genentechs-beginning-the-origins-of-biotechnology/ * 在一天晚上回家的车上冒出的idea，Roche paid 300 million for Cetus, 10,000 for bonus for Mullis * /article/506，sanger * GENOME PROJECTS: UNCOVERING THE BLUEPRINTS OF BIOLOGY * 1、与HGP项目组竞争 2、测序自己的全基因组，双倍体，仅次于wastion，产生人造生命 3、25000美元测序和用药，目标延长人类寿命 * /next-generation-sequencing.php * /watch?v=bFNjxKHP8Jclist=PL592D0AEB58337E73 * Nanopore, calibrate the raw data using machine learning * 内容延伸：人造生命 Synthia 1.0, 901 genes Syn 3.0, 475 genes Syn 2.0, 525 genes The human genome draft Nature. 2001 Feb 15; 409(6822):860-921 Science. 2001 Feb 16; 291(5507):1304-51 《从人类基因组到人造生命：克雷格·文特尔领路生命科学》 《测序人生》 Content 一、DNA的结构 二、早期测序方法 三、限制性内切酶与PCR 四、第一代测序技术 五、第二代测序技术 六、第三代测序技术 七、测序技术的应用 第一代测序技术回顾 测序策略的进化 Target DNA DNA fragments DNA fragments amplification Smaller DNA fragments DNA fragments amplification Smaller DNA fragments Sequencing Small DNA fragments Large amount DNA fragments DNA fragments amplification NGS DNA fragments amplification 五、第二代测序技术 Workflow Next Generation Sequencing High throughput DNA sequencing technique Employs Micro and Nanotechnologies Massive Parallel Sequencing Produce enormous amount of data 不同技术平台横向比较 测序仪的读长与数据量产出比较 五、第二代测序技术 2nd NGS platform Roche 454 (SBS) SOLiD (SBL) Complete Genomics/BGI (SBL) Ion torrent (SBS) Illumina (SBS) Roche 454 (2005, SBS) Shut down in 2013 SOLiD (SBL) Complete Genomics/BGI (SBL) Drmanac, et al. Science. 2010 cPAL Ion torrent (SBS) Cyclic Reversible Termination (CRT) Illumina (sequencing by synthesis, SBS) 150 million clusters! 测序策略的再进化 Target DNA DNA fragments DNA fragments amplification Smaller DNA fragments DNA fragments amplification Smaller DNA fragments Sequencing Small DNA fragments Large amount DNA fragments DNA fragments amplification NGS DNA fragments amplification Long DNA fragments NNGS 六、第三代测序技术 七、测序技术的应用 Waston, less than 1M 消费级测序：23 and me 消费级测序：WeGene 谢 谢！ * /article/dna_01 * /content/281/7/e7 * /content/281/7/e7 * venter * NGS与高通量测序技术 测序的用途 Angelina Jully