医学北大神经生物学11绪论.ppt

Hypnotic suggestions dissociate pain sensation and pain affect Rainville P et al. 1997, Science 277:968-971. Hofbauer RK et al. 2001, J Neurophysiol 86:402-411. 不同品系小鼠电针镇痛效果比较 Difference in analgesia of 2Hz EA 1.0-1.5-2.0mA on inbred strains of mice 129 A AKR B6 B10 B/c C3H C58 RI I I S SM 0 25 50 75 n=11-22 Strains I n c r e a s e o f P e a k % M P E ( E A o v e r C o n t r o l ) Difference in analgesia of 100Hz EA 1.0-1.5-2.0mA on inbred strains of mice 129 A AKR B6 B10 B/c C3H C58 RI I I S SM 0 25 50 75 n=11-22 Strains I n c r e a s e o f P e a k % M P E ( E A o v e r C o n t r o l ) Wan et al. Pain 2001 Pain threshold and the effect of 2 Hz EA analgesia in B10 and B6 mice -10 0 10 20 30 40 50 60 2 4 6 8 10 EA B10 EA NS Time （ min ） T F L （ s ） -10 0 10 20 30 40 50 60 2 4 6 8 10 n = 10 EA B6 EA NS Time （ min ） T F L （ s ） 1.0-1.5 mA Low responders High responders Hereditory difference between B6 and B10 mice ? H9 (chromosome X ) Igh2 (chromosome 12 ) Lv (chromosome 4 ) Festing, 1992 ? 59-67 cM segment on chromosome 4 McClive et al, 1994 ? 4 loci and 2 segments on chromosomes 2, 11, 13 and 16 Slingsby et al, 1995 Pain / analgesia-related genes in B10 and B6 mice Oprd1 δ OR (Chr 4 64.8 cM) Htr6 5-HT 6 R (Chr 4 64.9 cM) Htr1d 5-HT 1D R (Chr 4 66.0 cM) Htr1da 5-HT 1D α R (Chr 4 66.2 cM) Htr1db 5-HT 1D β R (Chr 4 66.2 cM) Adra1b NA α1b R (Chr 11 19.0 cM) Gabra1 GABA Aα1 R (Chr 11 19.0 cM) Gabrg1 GABA Aγ1 R (Chr 11 19.0 cM) Devi L. A. Oligomerization of Opioid Receptors 2002 Bioluminesence resonance energy transfer ( BRET ) technique for testing the distance between two molecules 100? 慢性疼痛的研究 韩济生 北京大学神经科学研究所 合作者： 张 旭 中科院神经科学研究所重点项目结题验收汇报 2003 年 2 月 22-24 日 Study on Chronic Pain Mechanisms Gene Peptide Protein In vitro In vivo Cognitive (DRG) Pain related 11 229 EST Novel Genes OFQ NST EM GDNF Mu OR Del