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i AS 5013.4—2009
ISO 4832:2006
Australian Standard®
Food microbiology
A
S
5
0
1 Method 4: Microbiology of food and animal
3
.
4
— feeding stuffs—Horizontal method for the
2
0
0 enumeration of coliforms—Colony-count
9
technique
PREFACE
This Standard was prepared by the Standards Australia Committee FT-024, Food Products
and Constituted Subcommittee FT-024-01, Food Microbiology, to supersede AS 5013.4—
2004, Food microbiology, Method 4: General guidance for the enumeration of coliforms—
Colony-count technique.
This Standard is identical with and reproduced from ISO 4832:2006, Microbiology of food
and animal feeding stuffs—Horizontal method for the enumeration of coliforms—Colony-
count technique .
The objective of this Standard is to give general guidelines for the enumeration of
coliforms. It is applicable to products intended for human consumption and for the feeding
of animals, and environmental samples in the area of food production and food handling, by
means of the technique of counting colonies after incubation on a solid medium at 30°C or
at 37°C.
Clauses 4 (Principle), 5 (Culture medium and dilution fluid) and 9 (Procedure) of
AS 5013.4—2004 have been technically revised.
The main changes are as follows:
(a) T
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