2015高通量测序专题培训：核酸序列分析.pdf

Nucleic acid sequence analysis Presented by Guangyong Zheng Outline Nucleic acid sequence analysis Famous database of nucleic acid sequence Analysis of nucleic acid sequence BLAST software  Basic concept of BLAST  BLAST use - web manners  BLAST use – local manners  Software demo PART I Famous database of nucleic acid sequence Database of nucleic acid sequence • Nucleic acid sequence is important material of bioinformatics research. • Nucleic acid sequences provide the fundamental starting point for describing and understanding the structure, function, and development of genetically diverse organisms. Database of nucleic acid sequence / Database of nucleic acid sequence Feature table /documents/feature_table.html Database of nucleic acid sequence /genbank/ Database of nucleic acid sequence http://www.ddbj.nig.ac.jp Database of nucleic acid sequence http://www.ebi.ac.uk/ena/ Database of nucleic acid sequence Submit sequence (/genbank/submit_types) Getting sequence from the GenBank database /nucleotide/ Getting sequence from the GenBank database Data format of nucleic acid (I) Getting sequence from the GenBank database Data format of nucleic acid (II) Getting sequence from the GenBank database Data format of nucleic acid (III) Getting sequence from the GenBank database /books/NBK25501/ Getting sequence from the GenBank database Getting sequence from the GenBank database PART II Analysis of nucleic acid sequence Analysis of nucleic acid sequence (1) Getting basic information of a nucleic acid sequence (2) Primer design (3) Tow sequences alignment (4) Multi sequences alignment (5) Finding open reading frame of a nucleic acid sequence (6) Gene prediction (7) Sequence localizatio