年产8万吨_α淀粉酶生产车间的设计（已处理）.doc

年产8万吨_α-淀粉酶生产车间的设计 8万t/a α-淀粉酶生产车间的设计 摘 要:本设计为年产80,000t α-淀粉酶的工厂设计,其通过枯草杆菌液体深层发酵、沉淀法提取达到分离纯化出菌体中α-淀粉酶的目的。本设计分别对α-淀粉酶的性质、用途、工艺流程及生产原理都做了相关的阐述,并对有关的物料和热量也作了相应的衡算,以及对标准设备的选型和计算,还对工艺指标、安全问题和环境保护都做了详细的阐述。通过设计得出结论:年产8万吨α-淀粉酶发酵工厂,共有18个500m3发酵罐,每月均放罐180罐,发酵周期为72小时,总提取率为82%,理论α-淀粉酶产量为27.8吨/罐,实际α-淀粉酶产量为31.03吨/罐。每月应投入生产总成本为3993万元,根据目前市场价格,年利润为6195.1万元。 关键词:α-淀粉酶;工厂设计;效益分析;发酵;发酵罐 Plant Design of Sixty thousand t/a α-Amylase Abstract:This project is designed by a factory which produces 60,000t α-Amylase a year.It achieves the aim of filtration and purification of the α-Amylase by using the deep ferment of hay bacillus and settling method.The design not only respectively illustrate the quality,use,technological process and production principle but also make a materials and heat balance,the type selection and calculation of the standard equipment,further more,illustrate the technic index,the problem of security and the environmental protection detailedly.conclusion made through the design:fermentation factory of 60,000t α-Amylase a year,it contains 35 fermentor of 500m3,The monthly discharge of liquid enzyme is 175 fermentors,The fermentation time is 120 hours,The recovery of liquid enzyme is 80%,The theoretic output is 27.8t per fermentor, while the virtual output is 31.03t per fermentor.It should monthly take the total product cost as 39.93 million yuan,the annual return is 61.951 million yuan, according to the current market price. Keywords:α-Amylase; Plant design; Profit analysis; Ferment; Fermentor 1 前言 毕业设计是普通高校本科教育的最后一个环节,也是最重要的一个环节,是理论知识和实际应用相结合的重要措施。本设计为年产60,000t α-淀粉酶的工厂设计,其通过枯草杆菌液体深层发酵、沉淀法提取达到分离纯化出菌体中α-淀粉酶的目的。 所有的生物体在一定的条件下都能产生多种多样的酶。因此,人们可以采用适宜的菌种,在人工控制条件的生物反应器中生产各种所需的酶。酶的生产方法主要有提取分离法、生物合成法和化学合成法等。生物合成法是在人工控制条件的生物反应器中,通过微生物细胞的生命活动合成所需的酶的方法,是当今应用最广泛的方法。 淀粉酶是水解淀粉酶和糖原酶类的统称,广泛存在于动植物和微生物中。淀粉酶amylase一般作用于可溶性淀粉、直链淀粉、糖元等,根据作用方式可分为α-淀粉酶与β-淀粉酶。α-淀粉酶α-amylase 又称为液化型淀粉酶,它作用于淀粉时,随机地从淀粉分子内部切开α-1,4糖苷键,使淀粉水解生成糊精和一些还原糖,所生成的产物均为α-型,故称为α-淀粉酶。α-淀粉酶广