临床医学论文没药倍半萜成分的分离鉴定及抗肿瘤活性.doc

临床医学论文-没药倍半祜成分的分离鉴定及抗肿瘤活性 【摘耍】 目的从没约提取物中分离纯化倍半话单体成分，并探讨其抑制肿瘤 细胞增殖的生物活性。方法没药石油瞇提取部分经硅胶柱层析分离得到二十多 个倍半祜类化合物。其中化合物1和2的结晶分别经熔点测定、薄层层析、1H NMR和13C NMR数据分析以及与已知化合物比较，确定其化学结构。利用 MTT法检测化合物1和2对人肠癌细胞HT 29和激素非依赖型前列腺癌细胞PC 3、PC 3M、DU 145及正常肝的永生化细胞L02细胞增殖的影响。结果 经 理化性质鉴定、波谱分析，确定化合物1的结构为〔l(10)E,2R,4R〕 2甲氧 基8,12环氧吉玛1(10),7,11三烯6酮;化合物2的结构为2甲 氧基5乙酰基4咲喃吉玛1(10)烯6酮，二者同属没药吉玛烷 型倍半祜类化合物。MTT结果表明，化合物1和2对大肠癌细胞增殖的抑制作用 较弱。而对三种激素非依赖型前列腺癌细胞的增殖有非常显著的抑制作用(P 0.01)，且呈剂量依赖性。另外，化合物对正常肝永生化细胞的增殖无明显影响。 结论经分离纯化、理化性质鉴定和波谱分析，确定了没药吉玛型倍半话化合物 1和2的结构，该化合物对前列腺癌细胞的增殖有明显的抑制作用。 【关键词】 没药；吉玛烷型倍半祜；前列腺癌；细胞株 To determine the structures of two sesqui terpene compounds isolated from Commiphora opobalsamum, and to investigate its thei r ant i pro! i ferat ion effect on tumor cel 1 growth. Methods Two sesqui terpenoids were isolated from the resinous exudates of Commiphora opobalsamum. Their structures were established on the basis of physical and chemical properties, and 1H NMR and 13Results Structures of two sesqui terpenoids were determined as (1( 10)E,2R,4R) 2 methoxy 8, 12 cpoxygcrmacra 1(10), 7, 11 trion 6 one and 2 methoxy 5 acetoxy furanogermacr 1(10) en 6 one. The results of MTT assay showed that both compounds had an inhibitory effect on the proli ferat ion of hormone independent prostate cancer cel 1 1ines in a concentration dependent manner. No significant anti proliferation act ivi ty was observed in colorectal cells exposed to these chemicals at low concent rat ions. Furthermore, less cytotoxici ty of these compounds was shown by using the immortal izied 1 iver normal cell line at concentrations tested. Conclusions The structure of two germacrane sesqui terpenoids were identified, and they can significantly inhibiI the proliferation of hormone independent prostate cancer ccl 1 growth. Key words: Myrrh ； Germacrane Sesqui terpenoids ； Prostate neplasms ； Cell lines 没药(myHh)为没药属植物树皮渗出的胶状树脂，为临床常用中药，具有 行气散血、消肿止痛等功效，有悠久的药用历史。国内外对没药化学成分和生物 活性硏究颇为关注，已分离鉴定了 300多个代谢产物，包括谄类、笛体、黄酮、 木脂素等化学成分。没药中含有丰富的倍半菇成分，其骨架类型多样，已发现吉 玛烷型檢烷型愈