合理的利用小鼠动物模型研究基因调节与病理发育.ppt

Mouse Model for Gene Regulation Studies ;Course Materials;Transgenic Technology;Part 1;What’s transgenic？;Importance of Transgenic Technology;Common Techniques Used for Making Transgenic animals;真核基因结构与调节;Gene Structure;Transgenic Construct;Promoter;Types of cDNAs;;Parts of transgenic construct;;（Founders）detection;Mouse Characteristics;Mouse Requirement for Transgenic Production ;Steps of making Transgenic mice;转基因鼠建立时间表;PMSF and HCG superovulation Donor egg，100－200 each time DNA concentration for injection: 2ng/ul Embryo transfer back to recipient: 20-30 PCR or Southern to detect founders, 15-50%, random insertion，copy 1 to over 100 First generation（F1) 0-100%，some may integrate at 2 cell stage, some may have more than one insertion locus Second generation，Mendel inheritance, 50％ Characterization, E10 to 5 months;Discussion;Transgenic Technology;Part 2：Gene Targeting;Importance of Gene Targeting;小鼠早期发育示意图;干细胞的特性;Targeting strategy;;;Steps for targeting;Chimera Mating;Time Table;输卵管移植;Comparison of Trangenic and targeting;(Gene Trapping);基因捕捉可以做为基因敲除的替代;Gene trap transgenic embryos;核移植技术与大动物克隆;转基因动物发展趋势;干细胞研究的进展与前景;核移植和大动物克隆的应用前景