hplc-elsd测定甘遂及其制剂控涎丸中大戟二烯醇的含量.docVIP

PAGE 5 PAGE 2 HPLC-ELSD测定甘遂及其制剂控涎丸中大戟二烯醇的含量 修彦凤1，2，施 贝1，朱程1 （1.上海中医药大学中药学院，上海 201203；2.南京中医药大学江苏省中药炮制重点实验室，江苏 南京 210029） [摘要] 目的： 建立甘遂及其制剂控涎丸中大戟二烯醇的含量测定方法。方法：色谱柱为Dikma Diamonsil C18柱（4.6mm×250 mm，5μm），流动相为甲醇-水（99∶1），流速为1.0 mL·min-1，检测器为蒸发光散射检测器，柱温为35 ℃。结果：大戟二烯醇在进样量为1.04～20.80μg的范围内(r=0.9996)，进样量的常用对数值与峰面积的常用对数值呈良好的线性关系，平均回收率为97.53%，RSD为2.56%，另外对10批甘遂饮片和5批控涎丸进行了测定。结论：所建立的方法简单、准确、重复性好，可用于测定甘遂 [关键词] 高效液相色谱-蒸发光散射；大戟二烯醇；甘遂饮片；控涎丸；含量测定 Determination of euphol in slices of Euphorbia kansui and KongXian pills by HPLC-ELSD XIU Yan-feng1,2, SHI Bei1, ZHU Cheng1 （1.College of Traditional Chinese Materia Medica, Shanghai?University?of?Traditional?Chinese?Medicine, Shanghai 201203, China;2. Key Laboratory of Processing Traditional Chinese Material Media（Nanjing?University?of?Traditional?Chinese?Medicine） Nanjing 210029, China） [Abstract]: Objective: To establish a method for the content determination of euphol in slices of Euphorbia kansui and its preparation KongXian pills . Method: HPLC-ELSD was carried out on Dikma Diamonsil C18（4.6 mm×250mm, 5μm）with methanol-water (99∶1) as mobile phase, the flow rate was 1.0 mL·min-1, and the detector was evaporative light-scattering detector(ELSD) , the column temperature was set at 35 ℃. Result: The calibration curve was linear in the range of 1.04～20.80μg( r=0.9996), the average recovery was 97.53％with RSD of 2.56％. the content of euphol in ten batches of slices of Euphorbia kansui and five batches of KongXian pills were determined. Conclusion: The method is simple, accurate and reproducible, it can be used to determine the content of euphol in slices of Euphorbia kansui and KongXian [Key words]: High Performance Liquid Chromatography-Evaporative Light-Scattering Detection(HPLC-ELSD); euphol; slices of Euphorbia kansui; KongXian pills ;content determination 甘遂在临床上多用于肝硬化腹水、胸腔积液、水肿及二便不利等症。生品仅限外用，内服必须炮制。以甘遂作为主药的常用方剂有控涎丸、十枣汤、甘遂散和甘遂半夏汤等。其代表性制剂控涎丸，收载于2005年版《中国药典》，由甘遂（醋制）、红大戟、白芥子组成。功效为涤痰逐饮。用于痰涎水饮停于胸膈，胸胁隐痛，咳喘痛甚，痰不易出，瘰疬，痰核。《中国药典》中对甘遂和控涎