电喷雾四级杆飞行时间Qtof理论.pptx

micrOTOF-Q 应用培训培训目的：了解ESI-Q-TOF的基本原理 掌握仪器的基本操作和维护 数据处理软件的使用潘晨松 博士布鲁克·道尔顿公司应用技术支持工程师培训安排第一天基本理论简介及使用中应注意的要点microTOF Control软件介绍(1)测样练习（tunemix、小分子、多肽、蛋白质）microTOF Control软件(2): Calibration; DataAnalysis 软件介绍(1)练习DataAnalysis软件的使用学员练习校正及测样第二天 复习及操作考查 HyStar LC/MS 练习小分子混合样品和蛋白/多肽样品测定 DataAnalysis软件(2) 练习处理LC/MS数据第三天 复习及操作考查软件介绍与练习（LibraryEditor, Isotope Pattern, Generate Molecular Formular, ReportDesigner…..) 练习处理LC/MS数据样品测定样品测定答疑、讨论液质联用型高分辨串联质谱仪 —— 电喷雾四级杆飞行时间 (ESI-Q-TOF)商品名：micrOTOF-QmicrOTOF-Q反射镜飞行管电喷雾离子源Collision Gas SupplyDry Gas HeaterHexapole四级杆QGlass CapillaryOrthogonal Accelerator检测器碰撞池去溶剂系统Ion ResolutionIon Transmission and focusIon Generation分辨离子离子传输与聚焦产生离子Ion Generation: Atmospheric Pressure Ionization (API)Atmospheric Pressure Ionization- Electrospray Ionization (API-ESI)Ion generation for MS analysis Nebulization Desolvation Coulomb explosions DesorptionUnder proper source conditions, individual ions only will enter the capillary. An unstable signal or capillary current may indicate a need for adjustment of gases, flow rate, or spray needle. See the User’s Manual for Troubleshooting tips. Generation of Ions - NebulizationDroplet formation in presence of electrical field at the needle tip within the spray chamberSampleNebulizer GasSpray NeedlegroundedDry GasDry GasHVCapillary ( 4 kV)Generation of Ions-DesolvationGeneration of Ions-Ion EvaporationGeneration of Ions - ESI sourceTo obtain spectral information, ions need to be created and introduced to the instrument for interrogationMust consider flow rates of Sample:NebulizerDry gasThe sample is introduced via the nebulizer. The ESI process is supported by nitrogen gas.The drying gas heats up the source and dries the spray.It acts as a counter current flow to the nebulized sample ensuring complete evaporation of the solvent in order to prevent droplets from entering the glass capillary.Generation of Ions – Desolvation unitThe desolvation unit contains the dry gas heating block and capillary housing. The g