研究生神经药理学jnc文章内容.pptx

Acute Administration of Fluoxetine Normalizes Rapid Eye Movement Sleep Abnormality, but not Depressive Behaviors in Olfactory Bulbectomized RatsWang YQ （王毅群）Fudan UniversityEmail: 061101069@fudan.edu.cnIntroductionOlfactory dysfunction is observed in patients with rapid-eye movement (REM) sleep behavior disorder.Olfactory bulbectomy (OBX) is one of the most preferred models of depression.EEG shows characteristic changes in depression as impaired sleep continuity, desinhibition of REM sleep and changes of nonREM sleep.Is there any causality between REM sleep abnormalities and depressive behavior?Materials and MethodsAnimals: Male SD ratsDrugs: FluoxetineOperation: OBX (AP: +8 mm, LR: ±2 mm, excision and aspiration of the OB)Implanted electrodes in brainOpen-field and forced swimming test High-performance liquid chromatography (HPLC)Normal olfactory bulb (OB)The Complete Removal of OB after SurgeryOBX ShamREMREMTime spent in each stage (min/h)NREMNREMAmount of each stage (min)* p＜0.05 and ** p＜0.01, n=7Increased REM Sleep in OBX RatsIncreased Episodes of REM Sleep, More Long REM Bouts in OBX RatsEpisode numberMean duration (s)Numbers of bouts* p＜0.05, n=7Increased Stage Transitions in OBX RatsTransition NumberEEG power density (%)* p＜0.05, n=7NREMNREMTime spent in each stage (min/h)Amount of each stage (min)REMREM* p＜0.05, n=5-8Sleep Rebound after 6 h Sleep Deprivation (SD) in OBX RatsN-methyl-3-phenyl-3-[4-(trifluoromethyl)phenoxy]propan-1-amineChemical Structure of FluoxetineShamOBXREM sleep (min/h)REM sleep (min/6 h)* p＜0.05 and ** p＜0.01, n=5-8Decreased REM Sleep after Acute Fluoxetine Treatment in OBX RatsNo Effect of Acute Fluoxetine Treatment on Depressive Behaviors in OBX RatsDistance Traveled (cm)Immobility Time (s)* p＜0.05 and ** p＜0.01 vs. vehicle-treated sham rats, n=4-5ng/g brain tissue wet weight* p＜0.05 and ** p＜0.01 vs. vehicle-treated sham rats; # p＜0.05 and ## p＜0.01 vs. vehicle-treated OBX rats, n=5-7Increased 5-HT, Decreased 5-HIAA Contents