果胶-壳聚糖复合水凝胶的制备及性能研究论文.doc

PAGE PAGE II PAGE I 题目：果胶-壳聚糖复合水凝胶 的制备及性能研究 果胶-壳聚糖复合水凝胶的制备及性能研究 摘 要 为了得到新的结肠靶向给药载体，制备了系列果胶-壳聚糖复合水凝胶。方法：以壳聚糖和果胶为原料，在不加交联剂及加入戊二醛(GA)或EDAC交联剂条件下，分别制备果胶-壳聚糖复合水凝胶。以体外释放度为指标，考察原料组成、溶液pH值、交联剂种类及用量等条件对该复合水凝胶溶胀性能的影响。进一步考察了复合水凝胶在模拟胃肠道生理环境下的溶胀性能及对模型药物牛血清白蛋白(BSA)的载药与释药性能。结果显示，在交联剂EDAC的作用下，果胶-壳聚糖(1:1，w/w)制得的复合水凝胶的溶胀度最小，在模拟生理环境下表现出良好的缓释作用，有望作为靶向缓释药物的载体材料。 关键词：果胶；壳聚糖；水凝胶；缓释作用 Study on Preparation?and properties of?chitosan composite?hydrogel system Abstract In order to obtain a new carrier for the colon-specific drug, chitosan/pectin composite?hydrogel was prepared. Chitosan/pectin composite?hydrogel?was prepared through the reaction between chitosan and pectin were used as raw materials, and Glutaraldehyde?(GA)?and EDAC?were?used as crosslinking agent. In vitro release as an indicator, discusses the changing pH of the solution, and other conditions affecting the selection and usage of different ratios of crosslinking agent (W/V) made of hydrogel swelling properties. The effects of hydrogel swelling properties in a particular buffer solution and the pH-stimuli-responsive. Bovine serum albumin (BSA) as a model drug, the possibility of doing drug delivery carrier. Experimental results show that the crosslinking agent is selected, the larger the content of the crosslinking agent and the pH influence on the rate of swelling of the hydrogel, and in the catalytic crosslinker EDAC hydrogel prepared maximum release rate (PEC: CEI=1:1 is ideal). Key Words：Pectin; Chitosan; Hydrogel; Release slowly PAGE PAGE V PAGE PAGE IV 目 录 TOC \o 1-3 \u 1 绪论 1 1.1结肠靶向给药系统研究现状 1 1.2天然多糖在医药学中的研究现状 2 1.2.1果胶 2 1.2.2壳聚糖 4 1.2.3果胶-壳聚糖复合水凝胶 5 1.3多糖基水凝胶研究现状 6 1.4水凝胶的结构与特性 7 1.5交联剂EDAC在化学反应中的作用机理 8 1.5.1 EDAC的性质 8 1.5.2 EDAC与果胶反应的交联机理 8 1.6本论文提出的背景及研究内容 9 1.6.1本论文提出的背景 9 1.6.2本论文的研究内容 10 2 实验部分 11 2.1试剂和仪器的表征 11 2.2果胶/壳聚糖复合水凝胶的制备 11 2.2.1不同组成比例果胶/壳聚糖(PEC/CHI)最佳配比的选择 11 2.2.2交联剂戊二醛作用下系列CHI/PEC凝胶的制备 12 2.2.3果胶与壳聚糖交联反应路线5% (W/V)的果胶与1% (W/V) 13 2.2.4交联剂EDAC作用下系列CHI/