以高通量方法筛选红桑叶的抗菌活性并进行活性组分的分离研究.doc

以高通量方法筛选红桑叶的抗菌活性并进行活性组分的分离研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：以高通量方法筛选红桑叶的抗菌活性并进行活性组分的分离研究 1 1 MATERIALS AND METHODS 4 2 RESULTS 7 3 DISCUSSION 9 文2：鳖甲抗肝纤维化活性组分的分析 11 1 材料与方法 12 2 试验结果 14 3 讨论 14 参考文摘引言： 15 原创性声明（模板） 16 文章致谢（模板） 17 正文 以高通量方法筛选红桑叶的抗菌活性并进行活性组分的分离研究 文1：以高通量方法筛选红桑叶的抗菌活性并进行活性组分的分离研究 Identification of the Antibacterial Activity of Morus rubra Leaves by a High-Throughput Screening Method Abstract:ObjectiveThis research intended to test the antibacterial activity of a plant extract library using a simple and fast colorimetric detection method and identify those plant extracts with bioactivity, and to further purify those bioactive fractio of the selected plant extracts. MethodsWe fit created a plant extract library and developed an antimicrobial High Throughput Screening (HTS) method. Then, the plant extracts were tested for antibacterial activity agait two species of bacteria, S. muta and A. actinomycestemcomita. ResultsThe Morus rubra leaf extract was selected because it had the strongest biological activity agait the oral bacteria tested. To further study the Morus rubra leaves, the extract was separated into small fractio via high-performance liquid chromatography (HPLC). The fractio were tested agait bacteria S. muta and A. actinomycestemcomita using HTS and the antibacterial activity of those selected bioactive fractio was further confirmed by blood agar plating method. One fraction of Morus rubra leaves, #31, exhibited near complete inhibiton (99% or better) for both bacteria, while two other fractio exhibited some, but not complete, inhibition of A. actinomycestemcomita. ConclusionThe HTS can be used to screen for a large plant extract library due to its fast and simple procedure. Morus rubra leaves exhibited the strongest antibacterial activity and the active fraction was obtained. The Morus rubra leaves have potential usage to treat bacterial infection in the future. Key wordsMorus rubra leaves； High-Throug