体外循环前后不同组织细胞因子的差异表达.doc

体外循环前后不同组织细胞因子的差异表达 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：体外循环前后不同组织细胞因子的差异表达 1 1 资料与方法 3 2 结 果 4 3 讨 论 5 文2：子痫前期外周血免疫相关基因的差异表达 8 1 对象与方法 9 2 结果 10 3 讨论 11 参考文摘引言： 13 原创性声明（模板） 14 文章致谢（模板） 14 正文 体外循环前后不同组织细胞因子的差异表达 文1：体外循环前后不同组织细胞因子的差异表达 Abstract: OBJECTIVE To determine the differentially expressed genes of cytokines between peripheral blood mononuclear cell (PBMC) and right atrium using gene chip technique. To explore the reactio to cardiopulmonary bypass(CPB) in the different The patients oxygenated bloods were drawn immediately before oet and termination of CPB. PBMC were obtained from heparinised blood by Ficoll gradient centrifugation. Right atrium were collected just before oet and termination of CPB. The differential expression between PBMC and atrium was compared using BD AtlasTM cDNA Expression Arrays. RESULTS Between PBMC and right atrium before CPB, the differentially expressed genes were interleukin 6, interleukin 13, Wnt5a, corticotropin releasing hormone receptor 1 (CRH1R), follicle stimulating hormone receptor (FSH-R) and discoidin domain receptor family, member 2 (discoidin domain receptor, DDR2). Both of the gene expression profiles changed after CPB. The differentially expressed genes between PBMC and right atrium were interleukin 6, interleukin 13, interferon alpha-inducible protein (clone IFI-6-16), S100 calcium binding protein A9 (calgranulin B), placental growth factor and Wnt5a. CONCLUSION The gene expression profiles in cytokines of PBMC and right atrium were different before CPB. The gene expression profiles changed in different manne after CPB. So, the differentially expressed genes changed. Key words： Cytokine; Gene expression; Gene chip; Peripheral blood mononuclear cell; Myocardium; Cardiopulmonary bypass 体外循环(cardiopulmonary bypass,CPB)手术中的创伤、低温、缺血-再灌注及血液与异物的接触触发了全身系统性炎症反应，其中细胞因子起传递介质的作用。生理状况下，同一种基因在不同的组织中表达量不同，对CPB反应也不相同[1-2];病理状态下，CPB后基因表达谱也不相同[3]。即使同一种细胞，来自于同一个体不同的生活环境，其基因表达也不同[4]。外周血单个核细胞(PBMC)是主