人参皂甙Rb1对应激大鼠肝脏热休克蛋白70和蛋白激酶C的影响及相关性研究临床医学.docVIP

人参皂甙Rb1对应激大鼠肝脏热休克蛋白70和蛋白激酶C的影响及相关性研究临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：人参皂甙Rb1对应激大鼠肝脏热休克蛋白70和蛋白激酶C的影响及相关性研究临床医学 1 1 材料和方法 3 2 结果 4 3 讨论 5 文2：人参皂甙对应激大鼠肝脏热休克蛋白70表达的影响 6 1 材料与方法 7 2 结果 9 3 讨论 10 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 13 正文 人参皂甙Rb1对应激大鼠肝脏热休克蛋白70和蛋白激酶C的影响及相关性研究临床医学 文1：人参皂甙Rb1对应激大鼠肝脏热休克蛋白70和蛋白激酶C的影响及相关性研究临床医学 Abstract： ［Objective］ To study the effect of gienoside Rb1 on liver heat shock protein70 and protein kinase C and the relatiohip between the two facto.［Methods］ 56 SD(Sprague Dawley) rats were divided into 3 groups: gienoside group, saline group, shamoperated group. Put these rats to death at 30min, 60min and 180min, extract liver and draw blood, use the ELISA to analyze the expression of HSP70 and the level of PKC.［Results］ (1) To compare the level of PKC at different time points of gienosides and the saline group,60 min gienoside group is higher than the saline group. There is a significant difference(P). Comparison of gienosides and saline 30 min and 180 min is no significant difference(P). (2)To compare HSP70 at different time points of gienosides and the saline group, 30 min and 60 min gienoside groups are higher than the saline group. There is a significant difference(P). Comparison of gienosides and saline 180 min is no significant difference(P). (3)The linear regression analysis of the level of PKC and the expression of HSP70 show that two facto are in significant positive correlation.［Conclusio］ Rb1 can increase the expression of HSP70. Rb1 can increase the level of movement of the expression of HSP70 and the level of PKC are in significant positive correlation. Key words: gienoside Rb1；stress；heat shock protein70；protein kinase C 应激是指机体在受到各种强烈因素(应激原)刺激时所出现的非特异性全身反应，又称为应激反应。研究发现应激过程中产生的多种介质、应激激素和细胞因子可以通过直接或间接途径导致肝细胞的损伤和功能变化，从而影响机体的应激调节功能，进而影响机体的预后或转归。笔者于2007年7月至2008年1月，采用大鼠肝脏缺血再灌注制作应激模型，通过检测大鼠肝脏热休克蛋白70（HSP70）和蛋白激酶C（PKC）的变化，观察人参皂甙Rb1对应激时对HSP70和PKC的影响及两者的关系，从而探讨人参皂甙Rb1保护应激机体的机制是否与PKC有关。 1 材料和方法 实验