植入式丝裂霉素控释剂对人肝癌裸鼠移植瘤增值、调亡及耐药性影响的研究.pdfVIP

上海医学 2002 年第 25 卷第 4 期 ·197 · ·肿瘤研究· 植入式丝裂霉素控释剂对人肝癌 鼠移植瘤 增殖、凋亡及耐药性影响的研究 任宁 　 全 　 　 　鲍 ( ) 　　【摘要】　目的 　探讨植入式丝裂霉素 MMC 控释剂对人肝癌 鼠移植瘤增殖、凋亡及耐药性的影响。方 法 　将不同剂量的 MMC 控释剂植入人肝癌 LCID20 小鼠皮下移植瘤内 ,与 MMC 腹腔注射组、硅胶载体植 ( ) 入剂组、生理盐水腹腔注射组对照 , 比较其凋亡指数、增殖细胞核抗原 PCNA 指数及 P糖蛋白的表达。结果 　 MMC 控释剂瘤内植入可明显抑制人肝癌 LCID20 小鼠皮下移植瘤细胞增殖 ,诱导肿瘤细胞凋亡 ,且不增加 肿瘤细胞的耐药性。结论 　丝裂霉素控释剂瘤内植入可能成为临床治疗中晚期肝癌的新方法。 【 】　丝裂霉素 ;细胞凋亡 ;增殖细胞核抗原 ; P糖蛋白 Influence on proliferation , apoptosis and drug resistance of hepatocellular carcinoma in nude mice by implantable controlled release mitomycin C 　　R EN Ning , W U Zhiquan , FA N Jia , et al. 　L iver Cancer Institute of Fudan U niversity , S hanghai 200032 　　【Abstract 】　Objective 　To appraise the expression of proliferation , apoptosis and drug resistance of hepatocellular carcinoma in nude mice by implantable controlled release mitomycin C. Methods 　Different dosage of implantable con trolled release mitomycin Cloaded microcapsules were implanted into highly metastatic models of human liver cancer in nude mice (LCID20) , and compared the effect on proliferation , apoptosis and drug resistance with conventional ad ministration method and placebo. Results 　Implantable controlled release mitomycin C could inhibit tumor prolifera tion , induce apoptosis , but not enhance drug resistance. 　Implantable controlled release mitomycin C may help pave the way for a new therapeutic modality in treatment of advanced liver cancer. ( Shanghai Med