HDAC6在ISO诱导大鼠心肌纤维化中的作用研究.doc

HDAC6在ISO诱导大鼠心肌纤维化中的作用研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：HDAC6在ISO诱导大鼠心肌纤维化中的作用研究 1 1 资料与方法 2 2 结果 4 3 讨论 6 文2：K252a对内毒素诱导急性肺损伤大鼠的保护作用研究 7 1 材料和方法 9 2 结 果 10 3 讨 论 11 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 13 正文 HDAC6在ISO诱导大鼠心肌纤维化中的作用研究 文1：HDAC6在ISO诱导大鼠心肌纤维化中的作用研究 Abstract：Objective The experiment was to investigate the expression of HDAC 6 in the rat model of myocardial fibrosis induced by SD rats were randomly divided into 2 groups，each group of 20 which to set up the normal group and model model group was treated with ISO daily by 5mg/kg for 7 d，the another group was given the same amount of its blood and myocardial tested its Heart quality was used to detected the content of type I and type III HE staining and Masson staining to observe the degree of myocardial expression of HDAC6 andα-SMA were tested by Western mRNA expression ofα-SMA and HDAC6 were examined by Compared with model group，heart mass index was increased（P）；HE staining and Masson staining showed collagen fiber hyperplasia in model group；Collagen type I and III in model group have increased relatively（P）；Western blot showed that protein expression in model group was higher than normal group（P），α-SMA expression is obviously higher than normal control group（P）；QRT-PCR detection showed that mRNA expression in model group was higher than normal group（P）.Conclusion HDAC6 in plays an important role in development and incidence of myocardial fibrosis。 Key words：Myocardial fibrosis；HDAC6；α-SMA 心肌纤维化是由较大程度的冠脉硬化性狭窄导致的反复持续性心肌缺血加剧所的结果，从而逐步发展成为心肌慢性缺血性心脏病。本实验研究的HDAC6作为II型HDAC家族的关键成员之一，在肿瘤的产生过程中起着有突出的作用，抑制相关活性的表达可以作为治疗恶性肿瘤相当重要的途径，然而对于其是否影响心肌重构中的重要步骤心肌纤维化罕有见闻。 1 资料与方法 材料 ：SPF级SD♂大鼠，200±20g，[实验动物使用许可证SCXK（皖）2011-002] ：异丙肾上腺素由上海禾丰制药有限公司提供；ELISA试剂盒购自北京北方生物技术研究所；HDAC6一抗美国Abcam公司提供；α-SMA、β-actin购自武汉博士德生物工程有限公司；二抗由中杉金桥生物技术有限公司提供，其余为分析剂。 方法 ：随机均分为两组，设立模型组和正常组，每组各20只。①模型组：皮下注射ISO溶液（5mg/kg-1·d-1），1次/d，持续不间断注射7 d；②正常组：皮下注射生理盐水溶液（5mg/kg-1·d-1），其他过程同模型组。 ：正常组和实验组在标准环境下喂食1w后处死，处死大鼠，腹