运用人多潜能干细胞研究SOX10在先天性巨结肠症中的发病机制.docVIP

运用人多潜能干细胞研究SOX10在先天性巨结肠症中的发病机制 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：运用人多潜能干细胞研究SOX10在先天性巨结肠症中的发病机制 1 1 资料与方法 3 2 结果 5 3 讨论 6 文2：成人巨结肠症2例 7 1 临床资料 8 2 讨论 9 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 运用人多潜能干细胞研究SOX10在先天性巨结肠症中的发病机制 文1：运用人多潜能干细胞研究SOX10在先天性巨结肠症中的发病机制 【Abstract】 Objective：To apply SOX10 human pluripotent stem cell research in the pathogenesis of hichsprung’s disease（HD）.Method：From January 2014 to December 2015，17 cases children with HD as a gender，age，matching of 17 cases of children with hichsprung surgery as the control group， collection of excision of the intestine， extracting RNA and detecting SOX10 expression pluripotent stem cells，directional differentiation into neural xiaohan cells， build SOX10 shRNA lentivirus plasmid，into SOX10 shRNA lentivirus plasmid，detect SOX10 gene expression after the downgrade，import SOX10，again to detect SOX10 gene expression，compared with the control group after raising，ability to observe neural xiaohan cell ：CPT samples，spas m，traitional period and the expaion period of SOX10/beta actni grey value were respectively （±），（±），（±） and the control group was （±），convulsion period and normal bowel SOX10/beta actni grey value comparison difference was statistically significant（）.25 μL SOX10 shRNA lentivirus cut SOX10 expression plasmid cut rate was %.Before joining migration facto laminin，fluorescence microscope counting random field of vision，according to the results of neural crest stem cell number count was 28%.After joining neural crest stem cell number count was 65%，the difference was statistically significant（ 字2=，P）.Conclusion：SOX10 in normal colon tissue increases，but lower expression in HD colon spasms segment，the imbalance in SOX10 expresses the occurrence and development of HD，experiment using human pluripotent stem cells confirmed by SOX10 can cause nerve xiaohan cell migration barrie，and promote migration factor laminin can restore its ability to migrate。 【Key words】 Human pluripot