疏肝理肺方防治应激条件下大鼠哮喘的作用机制.docVIP

疏肝理肺方防治应激条件下大鼠哮喘的作用机制 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：疏肝理肺方防治应激条件下大鼠哮喘的作用机制 1 文2：疏肝理肺方防治应激条件下大鼠哮喘的作用机制研究 1 1材料与方法 3 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 13 正文 疏肝理肺方防治应激条件下大鼠哮喘的作用机制 文1：疏肝理肺方防治应激条件下大鼠哮喘的作用机制 文2：疏肝理肺方防治应激条件下大鼠哮喘的作用机制研究 Protective and therapeutic effects of Shugan Lifei Recipe on rats with asthma under stress and the mechanisms ABSTRACT Objective: To explore the influence of psychological factor on asthma, the protective and therapeutic effects of Shugan Lifei Recipe on rats with asthma under stress and the mechanisms. Methods: Allergy and stressinduced asthma models were established in rats by giving ovalbumin and restraining stress. Radioimmunoassay, enzyme linked immunosorbent assay, in situ hybridization and immunohistrochemical method were used to detect plasma corticosterone, IL4, IFNγ, expression of glucocorticoid receptor (GR) mRNA in hippocampus and adrenocorticotropic hormone corticoliberin (CRH) positve neuron in hypothalamus. Optical and electron microscopes were used to observe the morphological changes of pulmonary hilar and hippocampal : Shugan Lifei Recipe (SGLFR) could reduce plasma corticosterone, decrease CRH positive neuro in paraventricular nucleus of hypothalamus, upregulate the expression of GR mRNA in hippocampus and reduce the injury of hippocampal neuron. SGLFR had certain inhibitive effect on hyperfunction of hypothalamopituitaryadrenal cortex (HPA) axis in rats with asthma under stress and could also relieve the pathological changes of the pulmonary hilar tissue. The level of plasma IFNγ was increased while the level of plasma IL4 was decreased in SGLFRtreated group. Conclusion: The mechanism of SGLFR in treating the rats with asthma under stress is probably to regulate the hyperfunction of HPA axis and the disorder of immuosystem. KEY WORDS stress; asthma; dispeing stagnated liver qi and regulating lung qi 支气管哮喘是一种多病因疾病，发病机制十分复杂，严重危害人类的健康。其发病受生物、心理和社会三方面因素的影响，其中精神心理因素在哮喘的发生、 发展 和转归中起着重要的作用。 现代 社会工作和生活节