实用生物医学实验技术-病毒制备与转染.pptVIP

The SeV vector stands unique among other vector systems in genomic reprogramming because it can express the reprogramming genes without chromosomal integration. SeV vectors can reprogram the nuclei of various human cells, including CD34+?cord blood cells, activated T lymphocytes and monocytes High efficiency… Application of SeV in Cellular Reprogramming * 精品课件资料 Lentivirus?(lente-, Latin for slow) is a?member?of Retrovirus?family, characterized by a long?incubation period. The?virions?are spherical and 80–100?nm in diameter, enveloped, with tiny?spikes?(about 8?nm) Lentiviruses can deliver a significant amount of?viral?RNA into the?DNA?of the?host cell?and being able to infect non-dividing cells. Lentivirus expression system is a useful research tool to introduce a gene product or shRNA into?cells or animal models for prolonged expression 慢病毒 (Lentivirus) * 精品课件资料 慢病毒结构 Genome ss-RNA, three main genes coding for the viral proteins in the order: 5′-gag-pol-env-3′ and two?regulatory genes, tat and rev Structural Protein The lentiviral proteome consists of five major structural proteins: Gp120?glycosylated?surface envelope protein SU, encoded by the viral gene?env. Largest 120 KDa. Gp41 glycosylated transmembrane envelope protein TM, also encoded by the viral gene?env. 2nd largest 41 KDa. P24 non-glycosylated?capsid?protein CA, encoded by the viral gene?gag. 3rd largest 24 KDa. P17 non-glycosylated?matrix?protein MA, also encoded by?gag. 4th largest 17 KDa. Non-glycosylated capsid protein NC, also encoded by?gag. 5th largest 7-11 KDa. * 精品课件资料 慢病毒表达系统 Lentivirus vector based on the human immunodeficiency virus-1 (HIV-1) has become a promising vector for gene transfer studies. The advantageous feature of lentivirus vector is the ability of gene transfer and integration into dividing and non-dividing cells. The pseudotyped envelope with vesicular stomatitis virus envelope G (VSV-G) protein broadens the target cell range. Lentiviral vectors hav