高通量测序培训班9-数据库验证与生物信息分析质控.pptx

数据库验证与生物信息分析质控 Han-Yu Chuang, PhD 莊涵宇 博士 Senior Manager, Bioinformatics and Genomic Applications Partnerships, Illumina Inc. © 2017 Illumina, Inc. All rights reserved. Company Overview: Illumina-at-a-Glance INDUSTRY …………………… PRODUCTS ………………….. Life Sciences Tools that help researchers understand the genetic basis of disease ULTIMATE PURPOSE ……... Improve human health by unlocking the power of the genome • Over 7000 Employee’s working in over 23 countries • Deloitte Technology Fast 500: 2001 – 2012 • Forbes Fastest Growing Tech Company – 2007, 2009 2010 • Product Innovation of the Year Award — Frost Sullivan • Fast 50 500 Winner — Deloitte Touche (2004-2007) • NASDAQ Top 100 Index • Red Herring’s top 100 Most Important Companies (2000) Top 50 Public Companies Most Likely to Change the World (2001) 2 More than a Decade of NGS Innovation 2007–2009 2010 2011 2012 2014 2015 2016 2017 2018 Genome Analyzer™ (1G and IIx) HiSeq™ 2000 MiSeq™ HiSeq™ 2500 HiSeq™ X NextSeq™ HiSeq™ 3000/4000 MiniSeq™ NovaSeq™ iSeq™ 100 Ten Introduced SBS Line scan imaging LED optics Fast fluidics Patterned flow cells 2-channel SBS Improved patterned flow cells Single optics module Optimized for scale and cost CMOS* sequencing Introduced PE module Miniaturized fluidics Fast line scanning Dry flow cell chemistry High cluster density Exclusion- Amplification Chemistry Integrated reagent cartridge Custom camera and laser 1 channel chemistry Extended applications 8 lane, random seeding flow cell BaseSpace™ connectivity Rapid Run Mode Miniaturized optics Real time analysis Onboard analysis Modular design New surface chemistry and dyes High speed scanning Modular design New compute architecture *complementary metal-oxide semiconductor (CMOS) 3 For Research Use Only. Not for use in diagnostic procedures. Illumina Sequencing-By-Synthesis Technology Robust Reversible Terminator Chemistry Foundation 3’ 5’ DNA (0.1-1.0 ug) A C G A T G C T T A C G A C G T A A G C C C G A T C T T Sample preparation Cluste