演示文档圆二色谱 Circular Dichroism( C D).ppt

圆二色谱Circular Dichroism (CD);Application;构 象;CD is very useful for looking at membrane proteins;主要内容;CD原理;圆二色性(circular dichroism, CD);圆二色性的表示;必威体育精装版文档;圆二色仪原理;蛋白质的CD谱;The peptide bond is inherently asymmetric is always optically active;蛋白质的CD谱;Near UV CD spectrum;必威体育精装版文档;Near UV CD spectrum of Lysozyme;;必威体育精装版文档;Far UV CD spectra of poly-L-Lys;CD signals for same secondary structure can vary (a bit) with environment;Best fitting procedures use many different proteins for standard spectra;估算蛋白质?螺旋含量;Limitations of CD secondary structure analysis;蛋白的三级结构;CD signal of a protein depends on its 2ndary structure;从CD谱分析蛋白质的结构类型;CD实验要点;Determination of Protein Concentration;Nitrogen flushing;必威体育精装版文档;HT plot;Bandwidth (SBW) selection;Number of data point;Accumulation;Sample concentration and cell pathlength;Typical Conditions for protein CD;溶剂的吸收!!!;必威体育精装版文档;Buffer Systems for CD Analyses;Summary;核酸的CD信息;圆二色谱在糖类化合物结构分析中的应用;