外源基因瞬时转化烟草叶片的研究.pdf

外源基因瞬时转化烟草叶片的研究 摘要：外源基因瞬时转化是当前生物学研究的热点之一，也是植物分子育种的常用手段。 烟草是外源基因瞬时转化的模式植物，可通过转基因手段获得转基因植株，为外源基因瞬时 转化植物叶片的研究奠定了基础。以本氏烟草作为实验材料，通过根癌农杆菌 EHA105 菌株 来介导，干涉本氏烟草的某些基因片段，此次实验用到转化方法是注射法，从而得到转基因 植株，并通过表达能力来分析外源基因对植物外源基因瞬时转化的影响，为基因功能的研究 起到了很好的作用。转化后对烟草进行选育，保存优良的转化植株，为烟草基因工程的研究 发展提供了便利，也为蛋白功能提供了一个便捷的技术。 关键词：瞬时转化；外源基因；烟草叶片；转基因 1 Transient Transformation Of Tobacco Leaves By Exogenous Genes Abstract: Transient transformation of exogenous genes is one of the hotspots in plant molecular biology and one of the bases of plant molecular breeding. Tobacco is a model plant of transient transformation of exogenous genes. Its research and the acquisition of mutant plants by transgenic means lay a foundation for the basic research of transient transformation of exogenous genes. The selected tobacco, mediated by agrobacterium tumefaciens EHA105 strain, interfered with the gene fragments of bens tobacco, transformed tobacco by injection to obtain transgenic plants, and analyzed the influence of exogenous genes on the instantaneous transformation of exogenous genes from the aspects of morphology, molecule and heredity, so as to provide a basis for the study of gene function. The polygenic transformation of tobacco grown in the application laboratory was preliminarily studied through transformation. Through the selection and breeding of tobacco strains, new tobacco strains are created, which provides convenience for the research and development of tobacco genetic engineering and a convenient technology for protein function. Key words: Instantaneous transformation ；exogenous gene ； tobacco leaf ； Transgene 2 目录 摘要1 Abstract 2 引言1