脐带血中CD34+、CUF-GM总数与TNC的线性分析.docxVIP

脐带血中CD34+、CUF-GM总数与TNC的线性分析 摘要 目的：探讨脐带血CD34+、CFU-GM（粒-巨噬细胞集落）与TNC（有核细胞数）之间是否存在线性关系。方法：收集山东脐血库供者合格筛选标准的脐带血1985例，分别进行细胞计数，CD34+检测与细胞菌落培养后，统计每例脐血的TNC、CD34+和CFU-GM 数值，最后进行线性分析。结果：1985例脐血TNC平均为（15.10±5.20）×108个，其生成的CFU-GM集落为（0.76±0.46）×106个，测得的CD34+细胞数为（3.97±2.89）×105个；CD34+细胞数、CFU-GM总数与TNC呈明显正相关（P0.01）。结论：可以通过CD34+和CFU-GM快速预测脐带血中造血干细胞的总数，TNC总数越高，脐带血中所含的造血干细胞数就越多，这为脐血的临床应用提供了重要的实验依据。??????? 关键词：脐带血；CD34+；CFU-GM；TNC??????? 中图分类号：Q2-33；R-33 文献标识码：A??????? Abstract Objective: To investigate whether there is a linear relationship between umbilical cord blood CD34+, CFU-GM(granulocyte macrophage colony) and TNC(total number of nuclear cells). Methods: 1985 Umbilical cord blood (UCB) units were suitable if the Shandong cord blood bank donor selection criteria strictly were fulfilled. The nucleated cell ( NC) number was counted by cell counter，CD34+ cells were measured by flow cytometry, and clonogenic assay was conducted at day 14 of culture．Finally, the linear relationship between umbilical cord blood CD34+, CFU-GM and TNC was assessed. Results: The average TNC of 1985 umbilical cord bloods was (15.10 ± 5.20) × 108, the CFU-GM was (0.76 ± 0.46) × 106, and the number of CD34+ cells was(3.97 ± 2.89) × 106: The number of CD34+ cells and the total number of CFU-GM were positively correlated with TNC(P 0.01). Conclusion: The total number of hematopoietic stem cells in umbilical cord blood can be rapidly predicted by CD34+ and CFU-GM. The higher the total number of TNC, the more hematopoietic stem cells contained in the umbilical cord blood. This provides an important experimental basis for the clinical application of umbilical cord blood.??????? Key words：Cord Blood；CD34+；CFU-GM；TNC??????? 作为造血干/祖细胞(hematopoietic stem cells/hematopoietic progenitor cells, HSCs/HPCs)的另一来源，脐带血已经越来越来的应用于临床治疗于治疗白血病，再生障碍性贫血，遗传性疾病、异常血红蛋白和肿瘤等多种恶性和良性血液疾病[1-4]。与骨髓和动员外周血来源的造血干、祖细胞相比有容易获得、移植成活率高、移植后移植物抗宿主病(graft-versus-host disease ,GVHD)的发病率相对较低等优势[5-6]，这让其在临床应用方面具有更广阔的前景。近几年，国家卫计委与国家食品药品监管总局更是规定干细胞制剂制