DB37_T 4030-2020苹果品种鉴定方法　微卫星方法.pdf

ICS 07. 080B 30/39DB37山 東东省地國方标准DB37/T4030—2020苹果品种鉴定方法微卫星方法Molecule identification method for apple strain2020-07-09发布2020-08-09实施山东省市场监督管理局发布 DB37/T 4030—2020PCR反应体系见表A.2.表A.2PCR反应体系试剂名称工作液浓度25μL反应体系加样体积/μLdLNP2. 5 mno1/L2 μ LPCR缓冲液10×2. 5 μ LTaq DNA 聚合酶5 U/ μ L0. 3 μ L上游引物10 pao1/μL0. 3 下游引物10 puo1/μL 0. 3DNA模板100 ± 50 ng2翅纯水17. 67 DB37/T 4030—2020部分苹果品种SSR基因型数据见表A.3。表A.3部分苹果品种SSR基因型数据品种果实或者SSR 位点SSSSR 位点SSR 位点SSR 位点SSR 位点SSR 位点SSR 位点SSR 位点茎枝19ZOH1O2TOHDCH02b03bCH02b10CH02d11CH02f06CH01d08LO9ZOHCH02g09CH02h11aCH03d07CH04d02CH05c07OTPFOHDAkagiFruiting128/144114/11679/95129/137119/121148/162275/275110/132111/120124/134185/199138/152153/153166/170168/191AkaneFruiting130/130114/12295/95129/141121/133148/162244/297130/132111/138107/124607/681126/152117/153166/174168/209AkibaeFruiting130/140112/11679/95125/129119/133148/162244/244110/112107/120132/134185/199126/152117/153166/170154/191Akita GoldFruiting144/144112/11662/6L125/135119/133142/148244/254112/132107/120132/134672/607126/126117/143166/170164/191Aodai3Rootstock146/148110/122101/16118/149127/133144/162244/260110/112138/152107/132160/223126/144117/127140/166166/176Baskatong0thers134/134126/138101/16121/139123/125152/152242/299104/110103/138140/146181/181126/134143/151162/166150/152BraeburnFruiting144/146112/11466/66123/161123/133154/154258/258110/110111/136132/136672/607126/126129/153166/166164/209ChinatsuFruiting130/134114/11495/99127/141133/133144/148254/297110/132111/120124/132607/607126/138LI1/2I1140/166166/168Cox sFruiting132/144110/11475/97123/135121/133154/158244/258110/12497/111107/132215/215126/126129/129168/199154/209 OrangePippinCripp sFruiting144/146112/11679/97125/129119/149148/148254/258118/124120/120132/132185/223126/126145/153170/174164/191PinkDiscoveryFruiting130/142114/114L6/96133/133121/133148/162244/297118/130107/111128/132183/203126/152117/143140/166164/164FiestaFruiting130/132110/11475/95123/129121/133154/162258/275112/12497/111107/132203/215124/126129/145168/189154/166FujiFruiting144/14