自诱导培养基.pdf

New Studier media for auto-induction and other applications Reference: F.W. Studier (2005) Protein production by auto-induction in high-density shaking cultures. Prot. Exp. Pur. 41, 207-234. ZYM-5052 medium for auto-induction It is recommended to use this medium for auto-induction. The amount of kanamycin (when appropriate) used should be 100 µg/ml. 958 ml ZY tryptone 1% yeast extract 0.5% 20 ml 50 x M Na HPO 25 mM 2 4 KH PO 25 mM 2 4 NH Cl 50 mM 4 Na SO 5 mM 2 4 20 ml 50 x 5052 glycerol 0.5% glucose 0.05% -lactose 0.2% 2 ml 1 M MgSO4 MgSO4 2 mM 0.2 ml 1000 x trace elements trace elements 0.2 x ZYP-5052 medium for auto-induction This medium should be used when stronger buffering is needed. The amount of kanamycin (when appropriate) used should be at least 200µg/ml. Better even would be 400 µg/ml. 928 ml ZY tryptone 1%