常用荧光染料的激发及发射波长.docx

实用标准文档 实用标准文档 文案大全 文案大全 荧光素名称 激发波长 发射波长 备注 (nm) (nm) 1,5 IAEDANS 336 490 1,8-ANS 372 480 4-Methylumbelliferone 385 502 5-carboxy-2,7-dichlorofluorescein 504 529 5-Carboxyfluorescein (5-FAM) 492 518 5-Carboxynapthofluorescein (pH 10) 512/598 563/668 Ratio Dye, pH 5-Carboxytetramethylrhodamine (5-TAMRA) 542 568 5-FAM (5-Carboxyfluorescein) 492 518 5-HAT (Hydroxy Tryptamine) 370-415 520-540 5-Hydroxy Tryptamine (HAT) 370-415 520-540 5-ROX 5-ROX (carboxy-X-rhodamine) 578 604 567 591 5-TAMRA (5-Carboxytetramethylrhodamine) 548 552 568 6-Carboxyrhodamine 6G 518 543 6-CR 6G 518 543 6-JOE 520 548 7-Amino-4-methylcoumarin 351 430 7-Aminoactinomycin D (7-AAD) 546 647 7-Hydroxy-4-methylcoumarin 360 449, 455 9-Amino-6-chloro-2-methoxyacridine 412, 430 471, 474 ABQ 344 445 Acid Fuchsin 540 630 ACMA (9-Amino-6-chloro-2- 412, 430 471, 474 methoxyacridine) Acridine Orange + DNA 502 526 Acridine Orange + RNA 460 650 Acridine Orange, both DNA RNA 440-480 520-650 Acridine Red 455-600 560-680 Acridine Yellow 470 550 Acriflavin 436 520 Acriflavin Feulgen SITSA 355-425 460 Aequorin (Photoprotein) 466 Photoprotein AFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFP 342441Alexa Fluor 430? 342 441 Alexa Fluor 430? 431 540 Alexa Fluor 488? 495, 492 519, 520 Alexa Fluor 532? 531, 532 553, 554 346 442 556, 557 556, 557 572, 573 577, 578 603 590, 594 617, 618 632 650 647 666 668 698 679 702 530-560, 580, 624- 580 645 530-560 580 630, 645 655, 660 345 445 345 425 347 444 353 442 555 655 346 442 350 445 345 425 347 444 600 360-381 446 630, 645 655, 660 625-650 755 466/380 520/530 Ratio Dye, Zn2+ 424 505 500 585 470 540 520 595 450 480 436 490 465 560 486 591 460 550 450 580 450-490 515 365 395 492, 503 520,528 482 520 430 550 409 447, 520 381, 382, 445, 447, blue shifted GFP Alizarin Complexon Alizarin Red Allophycocyanin (APC) AMC, AMCA-S AMCA (Aminomethylcoumarin) AMCA-X Aminoactinomycin D Aminocoumarin Aminomethylcoumarin (AMCA) Anilin Blue Anthrocyl stearate APC (A