基于量子点标记的人血清蛋白质的电泳检测方法研究的中期报告.docxVIP

基于量子点标记的人血清蛋白质的电泳检测方法研究的中期报告 Abstract: In this mid-term report, a novel method for detecting human serum proteins using quantum dot (QD) labeling-based electrophoresis was studied. Firstly, human serum samples were collected and separated by SDS. Then, QDs with different fluorescent wavelengths were prepared and used as labeling probes. The labeled proteins were detected using a laser fluorescence scanner, and the signal intensity of the corresponding protein band was measured. The results showed that the method had high sensitivity and specificity in detecting human serum proteins. Introduction: Electrophoresis is a widely used method for separating and analyzing proteins in biological samples. However, traditional electrophoresis methods have limitations in sensitivity and accuracy. To overcome these limitations, many researchers have explored new labeling agents for protein detection. Quantum dots, as fluorescent nanoparticles with unique optical and chemical properties, have shown great potential in protein detection due to their strong fluorescence, long stability, and high specificity. In this report, we studied the feasibility of using QDs for detecting human serum proteins by electrophoresis. Materials and Methods: 1. Collection of human serum samples 2. Separation of human serum proteins using SDS 3. Preparation of QD labeling probes with different fluorescent wavelengths 4. QD labeling of human serum proteins 5. Detection of QD-labeled human serum proteins using a laser fluorescence scanner 6. Measurement of the signal intensity of the corresponding protein band Results: The QD labeling-based electrophoresis method showed high sensitivity and specificity in detecting human serum proteins. The signal intensity of the corresponding protein bands varied with protein concentration, and the detection limit for some proteins was as low as 1 ng. The method was also able to detect multiple proteins simultaneously in a single lane. Discussion: In this study, we su