WYJ-2-DataSheet-生命科学试剂-MCE.pdf

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Inhibitors•ScreeningLibraries•Proteins

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WYJ-2

Cat.No.:HY-158058

CASNo.:3029403-05-9

分⼦式:C₁₇H₉F₂N₃O₄

分⼦量:357.27

作⽤靶点:Toll-likeReceptor(TLR);Pyroptosis

作⽤通路:Immunology/Inflammation;Apoptosis

储存⽅式:Pleasestoretheproductundertherecommendedconditionsin

theCertificateofAnalysis.

BIOLOGICALACTIVITY

⽣物活性WYJ-2是Toll样受体2/1(TLR2/1)的选择性激动剂，在⼈TLR2和TLR1瞬时共转染的HEK293T细胞中的

EC50为18.57nM。WYJ-2可诱导细胞焦亡(pyroptosis)，并对⾮⼩细胞肺癌(NSCLC)表现出抗癌活性[1]。

IC50TargetTLR2

体外研究WYJ-2(0-10μM)inhibitsheterodimerizationofTLR2andTLR1inTHP-1cells,stimulatestheadaptorprotein

myeloiddifferentiationprimaryresponseprotein88(MyD88)andthenactivatestheNF-κBsignalingpathway

withoutsignificantcytotoxicity[1].

WYJ-2(0-100μM)inhibitsproliferationincancercellsA549,HeLa,HepG2andMCF-7withIC50about10μ

M,inducescancercellpyroptosisbyactivationoftheNLRP3inflammasome[1].

WesternBlotAnalysis[1]

CellLine:PAM-differentiatedTHP-1

Concentration:0-10μM

IncubationTime:12h

Result:UpregulatedlevelsofMyD88,p-p65,TLR1andTLR2.