LCM纯化的鼻咽癌和正常鼻咽上皮组织蛋白质表达谱的建立的开题报告.docxVIP

LCM纯化的鼻咽癌和正常鼻咽上皮组织蛋白质表达谱的建立的开题报告

（Title）EstablishmentofproteinexpressionprofilesforLCM-purifiednasopharyngealcarcinomaandnormalnasopharyngealepithelialtissues.

（Abstract）Nasopharyngealcarcinoma(NPC)isatypeofheadandneckcancerwithahighincidencerateinSoutheastAsia.Theuseoflasercapturemicrodissection(LCM)topurifyNPCtissuesenablesthespecificanalysisoftumorcellswithoutcontaminationfromsurroundingnormaltissues.Inthisstudy,weaimtoestablishproteinexpressionprofilesforLCM-purifiedNPCtissuesandnormalnasopharyngealepithelialtissuesinordertoidentifydifferentiallyexpressedproteinsandpotentialbiomarkersforNPC.

（Introduction）Nasopharyngealcarcinomaisamalignanttumorarisingfromtheepithelialcellsliningthenasopharynx.IthasahighincidencerateinSouthernChina,SoutheastAsia,andNorthAfrica.Despiteadvancesinradiotherapyandchemotherapy,theprognosisofNPCremainspoor.Therefore,theidentificationofreliablebiomarkersfortheearlydetectionandprognosisofNPCisofgreatimportance.However,currentdiagnosticmarkersforNPClacksensitivityandspecificity,andthereisaneedfortheidentificationofnovelbiomarkersforimprovingthediagnosisandtreatmentofNPC.

LCMisatechniquethatenablestheisolationofspecificcellsortissuesfromcomplexsamples.InthecaseofNPC,LCMcanbeusedtospecificallycapturetumorcellsfrombiopsysamples,enablingtheanalysisofproteinexpressionprofilesofNPCtissueswithoutcontaminationfromsurroundingnormaltissues.BycomparingtheproteinexpressionprofilesofLCM-purifiedNPCtissuesandnormalnasopharyngealepithelialtissues,differentiallyexpressedproteinscanbeidentifiedaspotentialbiomarkersforNPC.

（Methods）Inthisstudy,wewilluseLCMtoisolateNPCtissuesandnormalnasopharyngealepithelialtissuesfrombiopsysamples.ThepurityoftheisolatedtissueswillbeassessedbyHEstaining