2 how to describe the growthof bacteriagrowth on agar plate exp2 report2如何描述细菌在琼脂平板上生长报告.pdfVIP

•Theappearanceofcolonialgrowthonagar

mediacanbeverydistinctiveforindividual

species.Observationofthenoticeable,gross

featuresofcolonies,thatis,oftheircolonial

morphology,isthereforeveryimportant.

•Thecolor,density,consistency,surfacetexture,

shape,andsizeofcoloniesallshouldbe

observed,forthesefeaturescanprovideclues

astotheidentityofanorganism,althoughfinal

identificationcannotbemadebymorphology

alone.

agarplate(numberand

characteristicsofcolony:size,form,

margin,pigment,hemolysis)

•Number:5coloniesobservedonagarplate

•Size:Big/small/medium(~2mmdiameter)

•Circular/round,raised,smoothsurface,white/golden-brown

color/colorless，withentiremargins.

➢Examplesofsomecolonialcharacteristicsonagarmedia：

Colonyappearanceof

Escherichiacoli

•Size:medium

•Pigmentation:Colorless

Opacity:Translucent

Form:Circular

Margin:entire

Surface:Smooth

Elevation:Raised.

Hemolysis

•:onbloodagarplate

•Hemolyticring/zone,

•betahemolysis,thedevelopmentofaclearzone

aroundabacterialcolonygrowingonbloodagar,

causedbythecompletebreakdownofthered

bloodcellssurroundingthecolonies.

characteristicofcertainpathogenicbacteria.

•alphahemolysis,thedevelopmentofagreenish

zonearoundabacterialcolonygrowingonblood

agar,characteristicofpneumococciandcertain

streptococciandcausedbythepartialposition

ofhemoglobin.

Hemolysis

alphahemolysisbetahemolysis

nohemolysis

如何描述琼脂平板上细菌的生长特征琼脂培养基上菌落生长的外观对于单个物种来说可能非常独特。因此，观察

菌