沉默lncRNA-PCA3减少前列腺癌细胞雄激素受体表达抑制前列腺癌细胞增殖和转移.docxVIP

沉默lncRNAPCA3减少前列腺癌细胞雄激素受体表达抑制前列腺癌细胞增殖和转移

[摘要]目的研究沉默lncRNAPCA3对前列腺癌细胞的影响。方法选择前列腺癌细胞LNCaP和C4-2细胞，使用siRNA干扰PCA3在细胞内的表达，实时定量PCR法检测干扰效果，检测siRNA干扰PCA3后对前列腺癌细胞增殖、迁移和侵袭能力的影响，Westernblot法在前列腺癌C4-2细胞内分别检测雄激素受体及其变体7的表达情况。结果PCA3特异性的siRNA可明显抑制前列腺癌细胞内PCA3mRNA的表达，抑制前列腺癌细胞增殖、迁移和侵袭潜能。沉默PCA3后的C4-2细胞内雄激素受体及其变体7表达明显减少。结论沉默lncRNAPCA3可减少前列腺癌细胞内AR及AR-V7的表达，从而抑制前列腺癌细胞增殖和迁移等恶性转变。

[关键词]长链非编码RNA;PCA3;siRNA;前列腺癌;雄激素受体

[中图分类号]R737.2?????[文献标识码]A?????[文章编号]1673-9701（2019）34-0021-05

EffectofsilencinglncRNAPCA3onreductionofprostateandrogenreceptorexpressionandinhibitionofprostatecancercellproliferationandmetastasis

YIXiaomin1，2??ZHANGJun1??LIANGYu1??SHENQunshan1??LIHaibo1

1.DepartmentofUrology，the901HospitalofPLA，Hefei??230031，China;2.DepartmentofUrology，theFirstAffiliatedHospitalofNanjingMedicalUniversity，Nanjing??210029，China

[Abstract]ObjectiveTostudytheeffectofsilencinglncRNAPCA3onprostatecancercells.MethodsTheprostatecancercellsLNCaPandC4-2cellswereselected，andsiRNAwasusedtointerferewiththeexpressionofPCA3incells.Theinterferenceeffectwasdetectedbyreal-timequantitativePCR，andtheeffectsofsiRNAonPCA3proliferation，migrationandinvasionofprostatecancercellsweredetected.Westernblotmethodwasusedtoinvestigatetheexpressionofandrogenreceptoranditsvariant7inprostatecancerC4-2cells.ResultsTheexpressionofPCA3mRNAinprostatecancercellsandtheproliferation，migrationandinvasionpotentialofprostatecancercellsweresignificantlyinhibitedbyCA3-specificsiRNA.Theexpressionofandrogenreceptoranditsvariant7inC4-2cellsaftersilencingPCA3wassignificantlyreduced.ConclusionSilencinglncRNAPCA3canreducetheexpressionofARandAR-V7inprostatecancercells，therebyinhibitingthemalignanttransformationofprostatecancercells，suchasproliferationandmigration.

[Keywords]Long-chainno